碩士 / 嘉南藥理大學 / 化粧品應用與管理系 / 104 / This study is to discuss how to extract fish-scale collagen from imidazole ionic liquids under different temperature and time. These eight liquids are made by combination reaction among imidazolium compound with different carbon-chain of organic acid and inorganic acid; such as nitric acid, acetic acid, sulfuric acid, hydrochloric acid, hydrobromic acid …etc.
To extract fish-scale collagen, our method is to wash up and then dry up the fish scales, and smash these scales with mechanical grinder. We can derive yellow dissolution from dissolving these scales with our ionic liquids at temperature 60℃, then we add cleaning fluid into the dissolution and obtain rough extract from it with high-speed centrifuge. Finally, we dry up the rough extract at room temperature and then obtain rough protein. From methods of calculating yield of proteins and electrophoresis of proteins, we can derive the distribution of molecular weight of proteins.
From above experimental results, we found 1-butylimidazolium bromide is the best ionic liquid to derive the rough fish-scale extract and collagen; yield of rough extract is 84.5% and yield of collagen is 77.7%.
| Identifer | oai:union.ndltd.org:TW/104CNUP0115020 |
| Date | January 2016 |
| Creators | LU, ZI-YAN, 呂姿燕 |
| Contributors | LEU, KUEN-LIN, 呂昆霖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 66 |
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