碩士 / 大葉大學 / 生物產業科技學系 / 104 / The traditional treatment processes for textile wastewater usually combined physical, chemical and activity sludge methods and it had high cost during operation. Biological treatments such as bacteria, fungi, and microalgae are a relatively inexpensive way to remove dyes from wastewater. Therefore, this study aims to development a technology which could remove the pollutant in the textile wastewater and accumulation the lipid in the algae simultaneously and investigate the cultivation under different conditions. In order to remove the pollutant in the textile wastewater and accumulation the lipid that can be transformed to fatty acid methyl esters (FAMEs) biodiesel fuel, two microalgal isolates were screened from seawater around Taiwan and then identified according to their ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) gene sequences and morphological characteristics. Two isolates with high lipid content were identified as Chlorella vulgaris Wu-G22 and Chlorella sp. Wu-G23. Furthermore the effects of pH and different of textile wastewater concentrations as well as phosphorus and nitrogen sources on immobilized and free-living microalgae cells and lipid accumulation using NH4+-N, COD and Color removal in the batch culture. The results show that the COD removal efficiency were more than 50% with aeration and pH of 7-10 for microalgae cells. While microalgae G22 was cultivated with aeration and pH 10, the total FAMEs content is highest (17 %). The effects of textile wastewater concentration (dilution 0% to 80%) and with/without aeration on microalgae growth and lipid accumulation in Chlorella sp. were investigated in this study. Adding K2HPO4 could enhance NH4+-N, COD and color removal removal efficiencies (>40%) for immobilized and free-living cells. The highest FAMEs content of up to 24% was obtained while free-living G22 strain was cultivated at aeration and adding K2HPO4 4 mg/L. Using different nitrogen sources, G22 and G23 could remove NH4+-N and COD with the efficnecy of 40% after 7 days cultivation. Adding (NH4)2SO4 as the nitrogen source with aeration conditions, total FAMEs content could reach >22% in free-living G22 microalgae. Studies on continuous cultivation of G22 and G23 under different hydraulic retention time (HRT). Therefore, the study feasibility of optimizing the environmental factor on simultaneous textile wastewater treatment and biodiesel production using free-living and immobilized Chlorella sp. cells.
| Identifer | oai:union.ndltd.org:TW/104DYU00111014 |
| Date | January 2016 |
| Creators | WU, SIN-YAN, 吳信諺 |
| Contributors | WU, JANE-YII, YEN,YUE-HORNG, 吳建一, 顏裕鴻 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 232 |
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