碩士 / 輔仁大學 / 生命科學系碩士班 / 104 / White spot syndrome virus (WSSV) is a serious pathogen that can infect almost all crustaceans, like penaeid shrimp, lobsters, and crabs. After infection, the mortality typically reaches 100% within 3 to 10 days and caused great economic losses. The horizontal infection of WSSV may be between the penaeid shrimp by contacting or eating with the infected shrimp or ingesting some crustacea organism which carry WSSV. It was confirmed that the crustacean copepods can serve as a vector in WSSV transmission and the Marsupenaeus japonicas juvenile were infected by WSSV-positive copepods. More serious is the commercial feeding organism “Artemia cysts”, had the positive signal of WSSV with the analysis of polymerase chain reaction. The aim of this study is to develop the sterile process for removing the WSSV which was adhered by Artemia, at the same time, the mortality of artemia must be maintained. Photodynamic treatment (PDT) is the selected sterile technique, it need a specific light source for exciting the photosensitizer and produce the reactive oxygen species (ROS) to inhibit the growth of microorganisms. In the preparatory experiment, cysts of Artemia were cultured for 14 days, then incubated with crude extract of WSSV for 6 hours to confirm the WSSV carried by Artemia, after analysis by PCR. Eighty six percent of Artemia adult has positive signals. The WSSV carried percent would down to 27.78% if infected Artemia were immersed with 0.1mM photosensitizer for 1 hour. Under the irradiation with 320mW/cm2 LED (660 nm) for 60 seconds, all of positive signals of WSSV were cleared. At the same time, the survival rate of experimental artemia still above 80%. These results showed that we had created the aquacultural PDT process which can fully remove the adhered WSSV on living Artemia.
| Identifer | oai:union.ndltd.org:TW/104FJU00105003 |
| Date | January 2016 |
| Creators | Chao-Yu Chen, 陳朝煜 |
| Contributors | Si-Shen Li, 李思賢 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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