碩士 / 高雄醫學大學 / 天然藥物研究所碩士班 / 104 / The current study focuses on natural product chemistry and bioactivity of Formosan zoanthid, Palythoa mutuki Haddon & Shackleton (Sphenopidae). The species of research materials was identified by the morphological and mt16S rDNA sequencing methods. Bioassay-guided fractionation of an ethanolic extract of P. mutuki collected in east coastal area of Taiwan has resulted in the isolation of two new ecdysones, palythones A and B (1 and 2), along with eighteen known compounds 3‒20. The structures of these compounds were determined by spectroscopic methods, especially 2D NMR analyses (COSY, HMBC, HSQC, and NOESY). The in vitro antiviral activities of isolated components 1‒13 and 15‒19 against dengue virus type 2 (DENV-2) were evaluated using the DENV infectious system. Palythone A (1) and ajugasterone C (4) exhibited potent antiviral activity with SI values > 36.7 and 69.7, respectively. In addition, the most active compound 4 was also tested for its EC50 value against DENV-1 (15.70 ± 2.36 μM), DENV-3 (9.48 ± 0.24 μM), and DENV-4 (12.15 ± 1.22 μM). These results suggested 4 is active for all dengue virus serotypes, especially serotype 3 (EC50 = 9.48 ± 0.24 μM). According to our antiviral data, the structure-activity relationships of isolated ecdysones against DENV-2 were established. Our marine natural product investigation suggested that ecdysones can be developed and served as drug discovery resources of anti-dengue virus agents. Besides, the structural diversity of the isolates was compared by using ChemGPS-NP computational analysis. Acoording to our mapping information, a specific quadrant was found to have anti-dengue virus activity.
| Identifer | oai:union.ndltd.org:TW/104KMC05068006 |
| Date | January 2016 |
| Creators | Shu-Rong Chen, 陳姝蓉 |
| Contributors | Yuan-Bin Cheng, 鄭源斌 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 171 |
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