碩士 / 國立中興大學 / 生物產業機電工程學系所 / 104 / This study aims to investigate the influence of microalgae bead cultivation under immobilization conditions and find out the main reasons of fugitive microalgae cells from alginate bead. First of all, the experiment investigated the relationship between alginate configuration methods and hardness of alginate bead. Next, the study analyzed the impact of the concentration of calcium chloride and alginate on generating time of alginate bead. This obtained results were also be used as the method of immobilization condition in the follow-up experiment. Third, the growth rate of microalgae, the fluorescence quenching, nitrogen removal rate and fugitive emissions rate of microalgae bead were measured to evaluate the effect of alginate concentrations on microalgae bead. Finally, the microalgae cells concentration inside of bead was measured and the position distribution of microalgae cells was observed to understand the reason of fugitive phenomenon.
The experimental results showed that (1) the configuration methods and the concentration of alginate had a significant influence on the hardness of alginate bead. The alginate bead was hardened with the increase of alginate concentration used. When the concentration of alginate was 1.0, 1.5, 2.0, 2.5 and 3.0%(w/w), the hardness of alginate bead was 1660, 2065, 2320 and 2409(g) respectively. (2) The decreasing of the concentration of calcium chloride also resulted in the delay of generating time of alginate bead. When the concentration of calcium chloride lowered from 0.3 to 0.1M, the generation time of alginate bead lengthened from 7 min to 10 min (3) The concentration of alginate would not affect the growth rate of microalgae and nitrogen removal rate. When the concentration of alginate was 1.0, 1.5, 2.0, 2.5%(w/w), the rate of nitrogen removal were all between 5.01 and 5.56 (ppm/day). However, the increasing concentration of alginate led to the delay of the fugitive phenomenon. Fugitive phenomenon was observed in the microalgae bead made from 1.0%(w/w) alginate on the 8th day of cultivation. In contrast, it was not observed in the one made from 2.5%(w/w) alginate until the 16th day of cultivation. The rising concentration of nitrogen amplified the chlorophyll fluorescence quenching. (4) Both overmuch number of microalgae cells in bead and overtime cultivation gave rise to the fugitive phenomenon. Additionally, the results demonstrated the positional distribution of microalgae cell are located on the surface of microalgae bead because of the restriction of light transmission.
The study ultimately used the chlorophyll fluorescence quenching as an indicator to estimate the temperature effect (-26, -14, 4 and 26 oC) on the long-term storage of microalgae bead. The experimental results displayed chlorophyll fluorescence quenching increased with decreasing storage temperature in aphotic, anhydrous and low temperature environment.. However, the microalgae bead could still survive for three days at 26oC. According to the results above, the immobilization method of microalgae bead has a great potential for future development, and the beads could repeatedly be used.
| Identifer | oai:union.ndltd.org:TW/104NCHU5415019 |
| Date | January 2016 |
| Creators | Chieh-Min Sun, 孫介民 |
| Contributors | Chung-Chyi Yu, 尤瓊琦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 74 |
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