碩士 / 國立成功大學 / 口腔醫學研究所 / 104 / In previous studies, we can see that there have been several studies about bone tissue regeneration composed of bone marrow mesenchymal stem cell, calcium-alginate, and growth factor. In this study, we aimed to use tissue engineering made up of bone marrow mesenchymal stem cell, barium-alginate, and growth factor to compare the alginate hydrogel integrity and osteoid secretion with calcium-alginate. First, we induced mesenchymal stem cell into osteoblast by osteogenic medium. Second, we observed the alginate hydrogel degradation by DMMB assay. Third, we calculated alginate hydrogel pore size and porosity by SEM and Micro CT. Fourth, we detected the cell viability within the alginate hydrogel by MTT assay. Finally, we observed cell morphology and osteoid secretion by SEM and EDS. The results show the degradation of barium-alginate is smaller than calcium-alginate, the pore size is 150~300μm, the porosity is greater than 95%, the cell can proliferate within barium-alginate through MTT assay, and the cell morphology within the hydrogel is round and cluster, and the calcium ion and phosphorus ions is detected by EDS. In summary, the composite of bone marrow mesenchymal stem cell and barium-alginate hydrogel can be a novel tissue engineering technique for bone
regeneration.
Key words : Bone marrow mesenchymal stem cell, barium-alginate hydrogel
| Identifer | oai:union.ndltd.org:TW/104NCKU5012014 |
| Date | January 2016 |
| Creators | Yen-KuoLin, 林晏國 |
| Contributors | Jehn-Shyun Huang, Fong-Yu Cheng, 黃振勳, 鄭豐裕 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 78 |
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