碩士 / 國立成功大學 / 化學工程學系 / 104 / In this study, rapid and direct Raman analysis of microalgae was proposed and demonstrated through microwells-assisted filtration using anodic aluminum oxide (AAO) membrane. Instead of evaporation, a droplet of algal solution was dispensed in the polydimethylsiloxane (PDMS) microwells, which was placed on top of the AAO membrane, followed by applying the vacuum. The algal cells deposited inside the microwells and were subsequently analyzed by Raman spectroscopy. The results showed that the algal cells were effectively concentrated and distributed quite uniformly inside the microwells, which led to uniform Raman signals among different algae pastes. Moreover, the time for preparing the algae paste was reduced to about 5 minutes. We also added HAuCl4 reagent to biosynthesize gold nanoparticles (AuNPs) and intended to enhance the Raman signals of the algal cells. The results showed that the lipid signals of the algal cells with biosynthesized gold nanoparticles were enhanced up to 20 times after algal cells being incubated in 10mM HAuCl4 for 15min. It was also found that carotenoids and chlorophyll may serve as reducing agents during reduction reaction.
| Identifer | oai:union.ndltd.org:TW/104NCKU5063073 |
| Date | January 2016 |
| Creators | Fang-YuWeng, 翁芳瑜 |
| Contributors | Yi-Je Juang, 莊怡哲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 120 |
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