碩士 / 國立成功大學 / 環境工程學系 / 104 / Cyanobacteria blooms and cyanotoxins have posed a great threat to human beings for many years, causing liver and renal pathology, mainly through oral intake of drinking water contaminated by the toxins. However, in a few recent studies, inhalation of aerosolized cyanobacteria and cyanotoxins is considered to be another pathway for exposure to cyanotoxins. Therefore, establishing a feasible and efficient detection method for the air samples will provide a better basis to evaluate the risk of inhalation exposure to cyanotoxins.
In this study, we developed sampling and analytical procedures and collected aerosols near eutrophic reservoirs and lakes in Taiwan, including two places in Taiwan main island and one in Kinmen island by PS-1 sampler, PM10 and PM2.5 cyclones. Enzyme linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR) methods were employed to quantify the aerosolized microcystins/cylindrospermopsins and four target genes within Microcystis and Cylindrospermopsis. The results show that, when 1% methanol was used to dissolve the dried cyanotoxins, recovery efficiency is ranged from 70% to 120% for ELISA. Besides, qPCR also had a good correlation with the cell enumerations in liquid phase, and therefore, the qPCR approach was further applied in determining the copy/cell number on the sampling filters.
The field sampling results show that Microcystis, Cylindrospermopsis and cylindrospermopsins were detected in the air samples collected near the studied reservoirs/lakes, with the gene copies of the two cyanobacteria ranging from below detection limit to 102 copies/m3 air and cylindrospermopsin from below detection limit to 0.02 ng/m3 air. This study demonstrates the presence of the harmful cyanobacteria and toxins near eutrophic reservoirs/lakes. However, more studies are required to better quantify the concentrations of the harmful cyanobacetria and toxins, and to link with the water quality of the reservoirs/lakes.
| Identifer | oai:union.ndltd.org:TW/104NCKU5515154 |
| Date | January 2016 |
| Creators | Pei-LingTung, 董珮伶 |
| Contributors | Tsair-Fuh Lin, 林財富 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 105 |
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