碩士 / 國立中央大學 / 生命科學系 / 104 / Endophytic fungi are microorganisms that live asymptomatically within plant tissues without causing apparent disease. Non-endophytic fungi grow on the plant surface, some of them may cause disease. Spinifex littoreus is an important coastal grass for stabilizing and protecting sand coastal areas and it has been shown to have anti-inflammatory properties, however, few has been published about the organisms associated with this plant. In this study, endophytic fungi were investigated by culture-dependent and culture-independent approaches and non-endophytic fungi were investigated by the opportunistic collection approach. By the culture-dependent and culture-independent approaches, 108 plant segments and 600 colonies yielded 19 and 12 species of endophytic fungi, respectively. Only 3 species were identical in both approaches, indicating that the combination of two approaches to detect endophytic fungi is more effective than a single approach. By the opportunistic collection approach, 7 species of non-endophytic fungi were identified; two of them were conspecific with endophytes. In this study, a new species of Neostagonospora was found by these approaches according to characterization by RNA polymerase II second largest subunit (RPB2) DNA sequences and micromorphology. The shape of conidiogenous cells as well as numbers of septa and size of conidia are useful characters for distinguishing between species of Neostagonospora.
| Identifer | oai:union.ndltd.org:TW/104NCU05105001 |
| Date | January 2015 |
| Creators | Jun-Wei Yang, 楊均威 |
| Contributors | Roland Kirschner, 羅南德 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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