碩士 / 國立嘉義大學 / 生物農業科技學系研究所 / 104 / Alginate is a polysaccharide isolated from brown algae. Previous studies showed that alginate could promote plant growth, increase crop yield, and act as an elicitor to trigger defense response against pathogen infection. Hence, alginate is used as a new type of plant growth regulator in agriculture for improving crop quality. In this study, alginate was tested in Arabidopsis to examine whether this polysaccharide can improve plant tolerance to abiotic stress. The results showed that the alginate treated seedlings exhibited higher degree of tolerance under high temperature and salinity. Application of alginate increased protein accumulation of alginate ascorbate peroxidase (APX) and superoxidase dismutase (SOD) also increased antioxidant enzyme guaiacol peroxidase (GPX) and catalase (CAT) activity, suggesting that this polysaccharide increased the ROS scavenging activity in the treated tissues. Real-time PCR was conducted to analyze transcription regulation of alginate in Arabidopsis. Our results showed that alginate induced the gene expression involved in biotic stress response, abiotic stress response, signal transduction, plant growth, ethylene biosynthesis and jasmonic acid biosynthesis. In Arabidopsis ethylene-insensitive (ein3), alginate-inducible genes were suppressed, suggesting alginate induced biotic and abiotic stress response through ethylene signaling pathway. Application alginate to Solanum lycopersicum promoted root growth; also increased antioxidant enzyme GPX and CAT activity and increased protein accumulation included APX, SOD, PR-2 and PR-3. In summary, our result showed that alginate is a potent inducer for plant growth and abiotic stress response.
| Identifer | oai:union.ndltd.org:TW/104NCYU5408010 |
| Creators | Yi-Chen Lin, 林宜蓁 |
| Contributors | Huey-Wen Chuang, 莊慧文 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 65 |
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