碩士 / 國立高雄師範大學 / 生物科技系 / 104 / Abstract
Soil algae have been found to be able to degrade and adsorb pollutants in soils. Recently, remediation of diesel-contaminated soils is an important issue. Thus, three stages of experiments were conducted. The first stage of experiments was to analyze the effects of algal addition on degradation of total petroleum hydrocarbon (TPH) under different nutrient contents and soil-turning frequency in order to determine the best conditions for bioremediation of diesel-contaminated soils. The second stage of experiments was to analyze the effects of algal addition in sterilized and unsterilized diesel-contaminated soils on degradation of total petroleum hydrocarbon (TPH) in order to identify the role of algae on the degradation of TPH. The third stage of experiments was to add algae into TPH-contaminated sites in order to determine the feasibility of using algae as biomaterials for bioremediation of TPH-contaminated soils.
The results in the first stage of experiments showed that the removal rates of TPH were in the order: the low-concentration groups (74%-88%)> the medium-concentration groups (29%-67%)> the high-concentration groups (10%-32%). Within the low-concentration groups, the best removal rate was found in the group with addition of algae and nutrients under two-week soil-turning and irrigation. Within the medium-concentration groups, the best removal rate was found in the group with addition of algae under four-week soil-turning and irrigation. Within the high-concentration groups, the best removal rate was found in the group with addition of algae under two-week soil-turning and irrigation. These results indicated that addition of algae increased the degradation of TPH. The results of denaturing gradient gel electrophoresis (DGGE) showed that abundance of bacteria was higher than that of algae, and addition of algae increased the growth of bacteria. The sequencing results showed that the diesel-contaminated soils contained added algae (i.e. Leptolyngbya sp.), TPH-degradation algae and bacteria. The major environmental factors influencing TPH degradation were pH, total phosphorous, soil water content, organic carbon, total nitrogen and toal algal numbers.
The results of the second stage of experiments showed that the removal rate of TPH in the group with algae-added sterilized contaminated soil (58%) was higher than those in the groups with algae-added unsterilized one (49%), with water-added sterilized and unsterilized ones (47%). It indicated that addition of algae increased the degradation of TPH. The DGGE results showed that abundance of bacteria was higher than that of algae. The sequencing results showed that the diesel-contaminated soils contained added algae (i.e. Fischerella ambigua and Leptolyngbya sp.), TPH-degradation algae and bacteria. Thus, it was inferred that added algae, and TPH-degradation algae and bacteria involved in TPH degradation processes.
The results of the third stage of experiments showed that after three-month treatment, the TPH removal rate in the low-contaminated site was 94% and that in the high-contaminated site was 75%. The DGGE results showed that added algae (i.e. Fischerella muscicola and Leptolyngbya sp.) were found in the both contaminated sites. It indicated that these added algae could grow in the TPH-contaminaed sites and might involve in TPH degradation. The TPH-degradation bacteria were also found in the TPH contaminated sites. These results suggested that both added algae and indigenous bacteria involved in TPH degradation processes. Thus, soil algae could be used as biomaterials for bioremediation of TPH-contaminated soils.
| Identifer | oai:union.ndltd.org:TW/104NKNU5111003 |
| Date | January 2016 |
| Creators | Sheng-Fong Huang, 黃勝豐 |
| Contributors | Chien-Jung Tien, 田倩蓉 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 215 |
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