碩士 / 國立屏東科技大學 / 水產養殖系所 / 104 / Arsenic is an element widely distributed in the environment and is often used in industrial products. While dissolved in the water, arsenic is absorbed and accumulated in algae. The algae food with excess amount of inorganic arsenic is hazardous to health. In order to ensure the food safety of algae, it is help to study the cultural factors that affect algal arsenic accumulation and to develop new cultural methods for low arsenic accumulation algae.
In this research, Sarcodia suieae of the Division Rhodophyta is the model algae species. It is cultured in sodium-arsenite-containing culture media with regulated cultural factors including temperature, illuminance, pH, culture time and concentration of phosphoric acid in media. Algae are collected and analyzed for arsenic form and concentration by inductively coupled plasma mass spectrometer (ICP-MS).
While the sodium arsenite (NaAsO2) concentration in culture media reaches over 1 mg L-1 for over an hour, algal photosynthesis is significantly inhibited. In each experimental group, the accumulated contents of total arsenic, arsenite and arsenate in Sarcodia suieae increase with sodium arsenite concentration in culture media. While cultured under temperature 25℃, illuminance 55 μmol photon m-2s-1 and pH 8, the highest accumulated values of total arsenic, arsenite, arsenate and dimethylarsonic acid in algae are as the following: 194.90±29.72, 66.41±4.00, 63.52±1.30 and 11.57±0.28 μg g-1. As cultured in seawater with 125 μg L-1 of sodium arsenite concentration for four weeks, the accumulated values of total arsenic and arsenite are 181.23±6.30及132.07±1.32 μg g-1, respectively; the amounts of which increase with culture time. As cultured in seawater with 500 μg L-1 of sodium arsenite concentration as well as 2 mg L-1 of phosphoric acid concentration, the total arsenic, arsenite and arsenate contents in Sarcodia suieae decrease by 64.9, 60.47 and 86.44% compared to the control group, while the accumulated dimethylarsonic acid content increases by 44.1% compared to the control group.On the other hand, the accumulated contents of Arsenocholine in Sarcodia suieae are not affected by the sodium arsenite concentration in media or by other cultural factors.
| Identifer | oai:union.ndltd.org:TW/104NPUS5086002 |
| Date | January 2016 |
| Creators | YeH, Siao-Yu, 葉曉瑜 |
| Contributors | Lee, Meng-Chou, 李孟洲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 92 |
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