碩士 / 國立中山大學 / 生物科學系研究所 / 104 / Genetic programs and signaling pathways are required for proper growth and patterning of blood vessels. Zebrafish is a powerful vertebrate model organism to study genetic control vascular development. In our previous study, transcription factors islet2 and coupTFIb regulated vein and ISV (intersegmental vessel) formation by notch signaling pathway. Microarray analysis showed that some GTPase related genes are changed in expression in isl2/coupTFIb MO. GTPase signaling has been shown important for angiogenesis, migration, proliferation etc. Here, we reported several novel GTPase related genes gtpbp1, sept8b, ect2 and rhoub that control vascular development in zebrafish. In-situ hybridization shows that gtpbp1l, sept8b, ect2 and rhoub mRNA is expressed in developing vessels, suggested the roles in vasculature. Loss of gtpbp1l, ect2 and rhoub by morpholino knockdown individually impairs the growth of ISV and CVP (caudal vein plexus), suggesting the role of these GTPase related genes in controlling ISV and CVP growth. On the other hand, we used overexpression strategy to test the role of GTPase related genes in vasculature. We found overexpression of gtpbp1l and sept8b results in vascular defects.
To test molecular mechanisms of vascular defects in gtpbp1l MO, ect2 MO, rhoub MO and overexpressing sept8b embryos, we examined the expression of vascular markers and we found the remodeling the expression of vascular markers in gtpbp1l MO, ect2 MO rhoub MO and overexpressing sept8b embryos. We also revealed the relationship between those GTPase related genes and Notch, VEGF, BMP signals. Together, we showed that GTPase related proteins gtpbp1l, sept8b, rhoub and ect2 play important roles for vascular development in zebrafish.
| Identifer | oai:union.ndltd.org:TW/104NSYS5112035 |
| Date | January 2016 |
| Creators | Han-lin Bao, 鮑翰霖 |
| Contributors | Chang-Yi, Wu, 吳長益 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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