碩士 / 國立中山大學 / 海洋科學系研究所 / 104 / The aim of this study was to investigate the sponge species diversity and sponge-associated microbial composition at a seawater aquafarm in southern Taiwan. During the period of May 2015 - February 2016, six demosponges and one calcareous sponge species were found, including Clathrina aff. beckingae, Pachastrella aff. fusca, Hymeniacidon aff. flavia, Esperiopsis sp., Dragmacidon sp., Chalinula cf. amoyensisc and Suberites sp. All of them were Taiwan’s new records. Five of them grew steadily at sites of S2, S3, S4, and S7. Among the sites, S7 had much higher concentrations of phosphate, nitrate and nitrite than all others. Through the examination on the 16S rRNA V3~V5 region by Next Generation Sequencing (NGS) tool, six major bacterial phyla were identified, including Proteobacteria, Bacteroidetes, Firmicutes, Actinobacteria, Cyanobacteria, and Fusobacteria. Based on the generic level comparison, Pseudoalteromonas, Thalassomonas, Sediminicola and Vibrio were more abundant in seawater samples. In contrast, Azospirillum, Rickettsia, Nisaea and Acaryochloris had higher amount in sponge samples. The results indicated that sponges had their unique microbiome. Besides, different sponge species from the same sites had similar microbial compositions. However, the process of relation-establishment between sponges and sponge-associated microbials still needs further research.
| Identifer | oai:union.ndltd.org:TW/104NSYS5276022 |
| Date | January 2016 |
| Creators | Yu-chun Lin, 林昱君 |
| Contributors | Tsung-Ming Chen, Li-Lian Liu, 陳琮明, 劉莉蓮 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 81 |
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