博士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 104 / Part I
Recent research indicated that the compounds from the crinoids inhibit the activation of the transcription factor nuclear factor-κB (NF-κB). However, to date, no study has been conducted to explore the bioactivity of anti-inflammatory and analgesic from the crinoid. Here we report the anti-inflammatory properties of comaparvin from the crinoid Comanthus bennetti based on in vivo experiments. Comaparvin isolated from crinoids significantly decreased the expression of inducible nitric oxide synthase (iNOS) protein and mRNA in lipopolysaccharide (LPS)-stimulated macrophage cells. Moreover, our results showed that treatment with comaparvin significantly inhibited mechanical allodynia, thermal hyperalgesia and weight-bearing deficits in rats with carrageenan-induced inflammation. Comaparvin also attenuated leukocyte infiltration and iNOS protein expression in carrageenan- induced inflamed paws. These results suggest that comaparvin is a potential anti-inflammatory therapeutic agent against inflammatory pain.
Part II
Burn injury is one of the most devastating injuries. This injury, even only local effect, may lead to long-term intense inflammation, tissue damage, infections, and increased mortality. Today, most of the drugs used in burn injuries mainly focus on the anti-microbial activity, and the course of burn injuries is not effectively improved by any topical agent yet. In the present study, we investigated the skin regenerative activity of C-phycocyanin (C-PC) through both in vitro and in vivo models, and the result showed C-PC did not enhance significantly the migration of EA.hy926 cells. Moreover, our results showed that C-PC enhanced the wound healing rate on burn wound but not on excision wounds in normal rats. Also, this ingredient did not improve the healing process of burn or excision wounds in diabetic rats as well. This result proposes that C-PC, through different mechanisms, might involve in skin regeneration in normal rats.
| Identifer | oai:union.ndltd.org:TW/104NSYS5277004 |
| Date | January 2016 |
| Creators | Li-Chai Chen, 陳立材 |
| Contributors | Zhi-Hong Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 176 |
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