碩士 / 國立中山大學 / 海洋生物科技暨資源學系研究所 / 104 / Recently, natural marine compounds have been considered as having potential for use in the development of novel anti-inflammatory drugs. They have been reported to have a broad spectrum of biological effects, including anti-inflammatory, anti-neuroinflammatory, and neuroprotective effects. Through preliminary screening, we previously found that marine-derived compounds, NRPG4-3 and NRPG07, isolated from Taiwanese soft coral, have anti-inflammatory effects. In the present study, to characterize the potential anti-nociceptive properties of NRPG4-3 and NRPG07, we evaluated NRPG4-3 and NRPG07 in a rat model of chronic constriction injury (CCI), a well-established model of neuropathic pain. We found that NRPG4-3 and NRPG07 significantly inhibit the neuropathic symptoms of thermal hyperalgesia and mechanical allodynia in a dose-dependent manner. Moreover, they also attenuated CCI-induced spinal neuroinflammation. Finally, we confirmed that administration of HO-1 inhibitor (ZnPP) attenuated the analgesic effect of NRPG4-3 and NRPG07 in rats with CCI. However, immunohistochemical analyses showed that NRPG4-3 and NRPG07 significantly inhibited CCI-induced activation of microglia, astrocytes, neuroinflammation, and reactive oxygen species (ROS) in the dorsal horn of the spinal cord. In conclusion, NRPG4-3 and NRPG07 are potential candidate compounds for the development of drugs to treat neuropathic pain.
| Identifer | oai:union.ndltd.org:TW/104NSYS5277007 |
| Date | January 2016 |
| Creators | Pei-Jyuan Li, 李佩娟 |
| Contributors | Zhi-Hong Wen, 溫志宏 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 159 |
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