碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / Brown macroalga, Sargassum horneri, and red macroalga, Gracilaria tenuistpitata were used as raw materials for dilute sulfated acid hydrolyzed polysaccharides. The optimum hydrolysis conditions and fermentable sugars contents of the acid hydrolysates were studied, as well as using four yeasts to produce the best bioethanol yields. It is found that 15% (w/v) alga powders, 4% (v/v) acid concentration at 121℃ in 60 mins resulted the highest amounts of fermentable simple sugars. HPLC was used to analyze the S. horneri and G. tenuistpitata acid hydrolysates contents, found the former that contained 1.77 ± 0.08 g/L of glucose, 1.7 ± 0.11 g/L of galactose and 2.86 ± 0.55 g/L of mannitol, the latter had 9.07 ± 0.3 g/L of glucose and 33.06 ± 1.6 g/L of galactose. Using four yeasts to ferment the simple sugars of the acid hydrolysates of S. horneri, found that inoculated mixing of 10% Pichia angophorae and 10% baker yeasts resulted the maximum ethanol yields of 1.66 ± 0.05 g/100g after 60 hs of fermentation at 30℃. And those of G. tenuistpitata that inoculated 20% thermotolerant yeast resulted the maximum ethanol yields of 6.72 ± 0.04 g/100g after 84 hs of fermentation at 40℃. The present study adopted dilute sulfated acid to hydrolyze algal polysacchrides to produce fermentable sugars and resulted great ethanol yields after yeasts fermentation, which is simpler and cheaper than those using expensive enzyme to hydrolyze algal polysaccharides, therefore the results of the present study could greatly reduce the cost to produce bioethanol from macroalgae.
| Identifer | oai:union.ndltd.org:TW/104NTOU5086001 |
| Date | January 2016 |
| Creators | Lin, Yu-Siou, 林昱秀 |
| Contributors | Chen, Yean-Chang, 陳衍昌 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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