碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / Sargassum oligocystum extract (SOE) incubated with white shrimp Litopenaeus vannamei hemocytes caused necrosis and released endogenous molecules, namely endogenous molecules Sargassum oligocystum extract (EM-SOE). Hemocytes incubated with EM-SOE caused degranulation, and changed percentage of large cells that deceased from 86% to 41%. Shrimp hemocytes incubated with EM-SOE caused increases in phenoloxidase (PO) activity and respiratory burst (RB). The immune parameters of white shrimp that received EM-SOE were examined after 24 h. The immune parameters including hyaline cells, granular cells, total hemocyte count (THC), PO activity, RB, superoxide dismutase (SOD) activity, and lysozyme activity increased significantly when shrimp received SOE and EM-SOE, compared to those of shrimp that received MS. Shrimp that received SOE and EM-SOE had increased phagocytic activity, phagocytic index and clearance efficiency to V. alginolyticus after 3 h. In another experiment, survival rate of shrimp that received SOE and EM-SOE after 24 h, and challenged with V. alginolyticus were significantly higher than that of control shrimp and then challenged with V. alginolyticus.A LC-ESI-MS/MS analysis indicated that EM-SOE contained damage-associated molecular patterns (DAMPs) including HMGBa, histone 2A (H2A), H2B, and H4, and other immune proteins like proPO I, Rab GPTase, and Rab 11 GPTase. In conclusion, EM-SOE induced by SOE contains DAMPs and other immune molecules, trigger innate immunity as evidences of hemocyte degranulation, change in cell size, and increases in PO activity and release of superoxide anion in vitro. Shrimp that received EM-SOE showed increases in immune parameters and increased resistance against V. alginolyticus infection.
| Identifer | oai:union.ndltd.org:TW/104NTOU5086016 |
| Date | January 2016 |
| Creators | Shi, Yin-Ze, 施寅澤 |
| Contributors | Chen, Jiann-Chu, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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