碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / The purpose of this study is divided into two parts: to record and compare the Centropyge acanthops with other marine angel fishs via record of embryonic ontogeny as well as larval and juvenile development; also, as the juvenile Pomacanthus maculosus grow up to know the effect of dietary protein and lipid on the growth performance.
In the first trial, among the species in Pomacanthidae, C. acanthops is the member of pomacanthide family, which has smaller size and the simlair about their morphological, reversible sex change and their spawn behavior. We hope the ability to rear this species in captivity is a valuable tool to be utilized for address a greater breadth and depth of biological questions.
Successful natural spawning of C. acanthops in captivity from 14 December, 2014 to 31 July, 2015 is described for the first time. Throughout the entire spawning period, the water temperature fluctuated between 25.0and 27.0 degrees. Fertilized egg were spherical and floating, with a mean diameter of 0.65 ± 0.05 mm, and a single oil globule with a mean diameter of 0.15 ± 0.05 mm. The eggs will hatch in 19 hours after fertilization under 28 degree. Newly-hatched larvae are 0.8-1.15 mm in length. The yolk sac is almost completely absorbed after three days post-hatch, when the larvae reach 2.3-2.5 mm. The larvae can feed biological feedings on euplotes and copepod nauplii enriched with Isochrysis galbana at first feeding. Bigger size of copepodites and small rotifers can be offered as the larvae grow. Photosynthetic bacteria and Bacillus subtilis was added to stabilize water quality. 12 DPH (5.2-5.4 mm TL), with light brown melanophores covered from head to cloaca and the gastrointestinal development completed.
In the second trial, white fish meal was used as dietary protein source, fish oilwas used as dietary lipid source, α-starch and cellulose was used as energy regulater. Five diets with different dietary protein inclusion levels of 36.77, 41.45, 47.05, 51.8, and 56.5% and dietary lipid levels with 9% are offered in experiment II for eight weeks to study the effect of different diets on growth performance of Pomacanthus maculosus junvenile. The weight gain of which fed diet with 47.05 % protein is the highest (68 %), and a model of weight gain against protein content fitted with broken-line regression shows the highest weight gain at 44.4 % protein level. Food conversion ratio (FCR) were 9.28-10.69. Specific growth rate (SGR) were 0.435-6.26. FCR and SGR were improve with dietary protein increase until the protein level 47.05% hightest.
In the third trial, white fish meal was used as dietary protein source, fish oil was used as dietary lipid source, α-starch and cellulose was used as energy regulater. Base on the previous experiment, junveniles P. maculosus were fed isocaloric diets containing five dietary lipid levels (5.7, 8.5, 10.2, 12.2, 15.1%) and 45 % dietary protein level for 8 weeks. The weight gain of which fed diet with 5.7 % lipid is the highest (103.33%) , is significantly higher(p < 0.05) than those fed treatment, without the 8.5% lipid ped contant.and a model of weight gain against lipid content fitted with second-order polynomial regression analysis shows the highest weight gain at 2.75 % lipid level. Food conversion ratio (FCR) were 2.70-4.89. Specific growth rate (SGR) were 0.698-1.268. FCR and SGR were improve with dietary protein increase until the lipid level 5.7% hightest.
| Identifer | oai:union.ndltd.org:TW/104NTOU5086017 |
| Date | January 2016 |
| Creators | Lee, Fang-Ren, 李芳仁 |
| Contributors | Nan, Fan-Hua, 冉繁華 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 63 |
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