碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / The aim of this study was to evaluate the effects of dietary antioxidant supplements extracted from algae (Spirulina sp., Chlorella sp., Nannochloropsis sp., Sargassum wightii and Ulva lactuca) on growth, immunity, antioxidant capacity and oxidative stress response of pacific white Shrimp (Litopenaeus vannamei). Twelve treatments were made including a Control (C), standard control astaxanthin (AX) and 10 diets using algae, five in single concentration Chlorella (Cx1), Spirulina (Sx1), Nannochloropsis (Nx1), Sargassum(Sx1) and Ulva (Ux1), and five with double concentration Chlorella (Cx2), Spirulina (Sx2), Nannochloropsis (Nx2), Sargassum(Sx2) and Ulva (Ux2). After orthogonal comparison, the treatments supplemented with raw extract and astaxanthin compared to control obtained significantly higher averages of MFW, MWG and SGR. Total hemocytes count (THC) showed to be significantly higher in supplemented diets compared to control under normal and hypoxia conditions. Analysis of non-stressed and stressed shrimp immune parameters ranked the greatest to the least as follow: RB under no stress: (Cx2 = Sx2 = Wx2 = Ux2 = Nx2) > (Sx1 = Cx1 = Wx1 =Ux1 = Nx1) > AX > C. RB under stress: (Ux2 = Nx2 = Wx2 = Ux2 = Nx2) > (Cx1 = Sx1 = Nx1 =Wx1 = Ux1) > AX > C. Superoxide dismutase (SOD), was very different and increased differentially under varying concentration of treatment supplementation. Glutathione peroxide (GPx), under stress was significantly different when comparing the supplemented diets against control. However, supplemented diets were no significantly different, despite, source or concentration. Glutathione reductase (GR), similarly to GPx under stress was significantly higher when comparing the supplemented diets against control and astaxanthin. The orthogonal analysis for the metabolic response showed, Gluc and Trigs non-stressed and stressed condition had significant difference in three levels of comparison control vs. treatments, astaxanthin vs. algae extracts and algae extracts x1 vs. x2. Hypoxia test revealed that double concentration diets lasted for longer time under stress conditions and were significantly higher than single concentration of supplemented diets. Overall, results in this study showed that immune response, antioxidant response and metabolic response of L. vannamei, were enhanced significantly by the supplementation of algae-derived antioxidant in their diets.
| Identifer | oai:union.ndltd.org:TW/104NTOU5086024 |
| Date | January 2016 |
| Creators | Luis Fernando Valenzuela Munguia, 范尼思 |
| Contributors | Yew-Hu Chien, 陳瑤湖 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 79 |
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