碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 104 / The effects of laminarin and sodium alginate on the haemocytes of white shrimp Litopenaeus vannamei that reared at different salinity levels of 35‰, 25‰, 15‰, and 10‰ were examined in vitro. Shrimp haemocytes incubated in laminarin or sodium alginate were examined for degranulation, cell size, cell viability, necrosis, phenoloxidase activity (PO), and respiratory burst (RB). Shrimp haemocytes respectively incubated with 1 mg ml-1 laminarin and sodium alginate for 30 min caused more degranulation and necrosis at 35‰, 25‰, and 15‰ than those of shrimp that reared at 10‰. Shrimp haemocytes received laminarin caused reduction in the percentages of large cell and viable cell, and the reduction increased directly with laminarin that incubated with shrimp haemocytes, but negatively related to salinity level. Shrimp haemocytes received sodium alginate caused reduction in the percentages of large cell and viable cell, and the reduction increased directly with concentration of sodium alginate that incubated with shrimp haemocytes, but negatively related to salinity level. The PO activity and RB of shrimp increased directly with salinity level. PO activity and RB of shrimp haemocytes, incubated respectively with laminarin and sodium alginate at 1 mg ml-1 and 3 mg ml-1 significantly increased compared to the controls. PO activity and RB were significantly lower in shrimp that reared at 10‰, compared to those of shrimp that reared at 15‰, 25‰, and 35‰. It is concluded that haemocytes of shrimp that reared at 10‰ receiving sodium laminarin or sodium alginate exhibited low activation of immune response as evidenced by less degranulation, less necrosis cell, low percentage of large cell and low percentage of viable cell as well as reductions in PO activity and RB. The immune response of shrimp receiving laminarin or sodium alginate is considered much better and earlier in shrimp that reared at high salinity (35‰, 25‰, 15‰), compared to those of shrimp that reared at low salinity (10‰).
| Identifer | oai:union.ndltd.org:TW/104NTOU5086036 |
| Date | January 2016 |
| Creators | Nuri Muahiddah, 莫如里 |
| Contributors | Jiann-Chu Chen, 陳建初 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 10 |
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