碩士 / 國立臺灣海洋大學 / 食品科學系 / 104 / Diethylstilbestrol is a synthetic oestrogen. It was once widely used as a growth promoter for food producing animals. Its residue in food may cause human endocrine disorders, moreover, the canceration of hormone-dependent organs. Therefore, diethylstilbestrol has been banned on food-production animals all over the world. The purpose of this study was to establish a high-performance liquid chromatography-ultraviolet detection (HPLC-UV) method for analyzing diethylstilbestrol residue in food. It included the search of optimum conditions for high-performance liquid chromatography, extraction of sample, and solid-phsae extraction.
Samples including chicken liver, pig liver, pig brain, and shrimp were found to have higher extraction yields by using microwave-assisted extraction at the power of 100W for 2 minutes than that using ultrasound-assisted extraction for 30 minutes. The recoveries were between 74.9 and 122.2%.
In determining the amount of diethylstilbestrol in the samples mentioned above, an InerSistain® column was used along with a UV dector detecting at 240 nm. A mixture of acetonitrile and water at the ratio of 55:45 (v/v) was used as the mobile phase for isocratic elution. Out of the 6 samples determined, only a sample from a fishing shrimp farm at Xindian was found to have residual diethylstilbestrol at the amount of 2.8 ± 0.2 μg/g.
| Identifer | oai:union.ndltd.org:TW/104NTOU5253004 |
| Date | January 2015 |
| Creators | Cheng, Ya-Ping, 鄭雅屏 |
| Contributors | Hung, Lang-Bang, 洪良邦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 71 |
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