碩士 / 國立臺灣海洋大學 / 輪機工程學系 / 104 / Oils produced by microorganisms also known as single cell oils (SCOs) compared to animal and vegetable oil have shorter growth cycle and higher oil productivity, unaffected by climate and no controversial issue of food competition, thereby have the potential for applications in producing biofuels. In consideration and of the current dilemma of producing microalgae biofuels, microalgae (Nannochloropsis oculata), heterotrophic photosynthetic bacteria (Rhodobacter sphaeroides) and chemical heterotrophic bacteria (Bacillus subtilis) were selected for co-cultivation. During the cultivation, the variations of the biomass, particle size, nitrate content, phosphate content, and lipid content were measured and analyzed to develope an effective and economical microalgae cultivation system.
The Experimental results show that co-cultivation of Nannochloropsis oculata with Bacillus subtilis for 72 hours can achieve microorganisms biomass 220.953mg/ L and lipid content 20.1mg/ L. After 72-hour of co-cultivation Nannochloropsis oculata with Rhodobacter sphaeroides, the microorganisms biomass and lipid content achieve 243.004mg/ L and 24.1mg/ L, respectively. Hence, co-cultivation of Nannochloropsis oculata with Rhodobacter sphaeroides were more productive than Bacillus subtilis. Furthermore, the microorganisms biomass of co-cultured Nannochloropsis oculata with Rhodobacter sphaeroides were 1.28 times of single culture, and lipid content were 1.36 times of those of being culture alone. In condequence, adding Rhodobacter sphaeroides to Nannochloropsis oculata can enhance the microorganisms biomass and lipid content of the microalgae.
| Identifer | oai:union.ndltd.org:TW/104NTOU5484002 |
| Date | January 2016 |
| Creators | Chia-Hsin Yang, 楊嘉欣 |
| Contributors | Cherng-Yuan Lin, 林成原 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 41 |
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