碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 104 / Abstract
Some diatoms can produce polyunsaturated aldehydes (PUAs), which are a kind of secondary metabolites, to induce self-autolysis via NO or ROS mediated pathway when ambient environment is stressful. However, regulatory mechanism mediated by PUAs in diatoms is still unclear. In addition, previous works indicated that mRNA expression of the death specific protein (DSP), which was suggested to participate in the process of programmed cell death (PCD) in centric diatoms, was regulated by ROS or NO signaling. But, the function of DSP in pennate diatoms is not characterized so far. Therefore, the aim of this study is to clarify whether PUA signaling can regulate DSP expression in pennate diatoms. Phaeodactylum tricornutum was chosen as a model organism because it is not a PUA producer. They were first treated with three different kinds of PUA (heptadienal, octadienal, decadienal), H2O2 (as ROS donor) and diethylamine nitric oxide (as NO donor) sepeartely, and then the levels of PtDSP mRNA and its protein were detected using quantitative reverse transcription PCR (Q-PCR) and weastern blot. The results showed that NO or ROS induced significant mRNA expression of PtDSP. Moreover, only decadienal was able to induce PtDSP mRNA expression. These high expressions were suppressed when the diatoms were pretreated with Tempol (ROS scavenger) or c-PTIO (NO scavenger). These results implied that under stressful environment, P. tricornutum can sense ambient PUA released from other diatoms and then induce PtDSP expression via ROS or NO mediated regulatory pathway.
Keywords: diatom, death specific protein, polyunsaturated aldehyde, nitric oxide, reactive oxygen species
| Identifer | oai:union.ndltd.org:TW/104NTOU5613003 |
| Date | January 2016 |
| Creators | Liu, Tai-Yu, 柳泰宇 |
| Contributors | Chen, Shiow-Yi, Chung, Chih-Ching, 陳秀儀, 鍾至青 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 44 |
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