碩士 / 國立臺灣海洋大學 / 生命科學暨生物科技學系 / 104 / Obesity has emerged as a global health problem with more than 19 billion adults to be classified as overweight or obese, but also medically important global issue, when excess fat accumulation, and causing negative health effects. If excessive fat accumulation and for many negative health effects like diabetes, heart desease and cancer risk. From previous studies the mir27b may be to PPARγ non-coding regions on the action site, and through the mir27b damage will affect PPARγ and C/EBP calibration. Therefore, we chose MicroRNA-27b as the major research goals through RNA-sponge technology, combined with the same sequence number of miRNA in synthesis, research for specific binding miRNA. From the study found that in mir27b in the Zebrafish, obvious difference for fat build. In theory, mir27b sponge may induce activation of PPARγ, and generate more fat cell differentiation.In Oil o stain the study proved, fatty feeding mir27b sponge transgenic fish oil droplet of distribution is higher than wild type and gives it a significant difference. Beside the RT-PCR to half-quantitative mir27b sponge is able to do transgenic fish the PPARγ and C/EBP be released aim to fat metabolism in connection with trends, mir27b is adipose metabolisms and plays an important role build in him, so that fat metabolisms. Finally, we hope our model fish can provided a platform to study lipid accumulation and early stage adipocyte differentiation.
| Identifer | oai:union.ndltd.org:TW/104NTOU5613005 |
| Date | January 2016 |
| Creators | Chen, Hsuan-chih, 陳宣智 |
| Contributors | Her, Guro-Mour, 何國牟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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