碩士 / 國立臺灣大學 / 農業化學研究所 / 104 / Plant non-specific lipid transfer proteins (nsLTPs) are basic proteins, which have the ability to bind or transfer various types of hydrophobic molecules in vitro, such as fatty acids, fatty acyl-CoA, glycolipids, phospholipids and cutin monomers. Rice LTP is a large gene family consisting of 52 nsLTPs genes. Our previous results have revealed that the expression of OsLTP2 was notably increased in glutathione reductase 2-RNAi and ascorbate peroxidase 8 rice mutant as compared to wild-type (WT) under salt stress. In this study, OsLTP2 overexpression (OsLTP2-OE) and RNA interference (OsLTP2-RNAi) transgenic lines were used to study the physiological roles of OsLTP2 in rice. Results showed that knockdowned OsLTP2 mRNA by RNA interference lead to reduction of plant height (31%), fresh weight (23%), dry weight (25%) and yield as compared to WT, whereas no difference was observed between WT and OsLTP2-OE. Reduced expression of OsLTP2 caused a reduced cell size in leaf sheath of seedlings, abnormal grains with high chalkiness. Simultaneously, expression of abscisic acid biosynthetic genes, 9-cis-epoxycarotenoid dioxygenase (OsNCED3, OsNCED4, OsNCED5)、ABA signaling genes including OsABI3 and OsABI4 and ABA responsive gene OsRab16a were induced. However, the ABA catabolic gene, rice ABA 8’-hydroxylase (OsABA8ox3) was suppressed, leading to an increased levels of abscisic acid in shoots of rice seedlings. These results show that OsLTP2 may participate in the regulation of rice growth, development and abscisic acid biosynthesis. Nevertheless, OsLTP2-RNAi showed an increased concentration of abscisic acid in shoots of seedlins, but the tolerance to salt stress was not able to find significant difference as compared to TNG67 and OsLTP2-OE.
| Identifer | oai:union.ndltd.org:TW/104NTU05406004 |
| Date | January 2016 |
| Creators | Cheng-Han Hsieh, 謝政翰 |
| Contributors | Chwan-Yang Hong, 洪傳揚 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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