碩士 / 國立臺灣大學 / 農業化學研究所 / 104 / As the wounds appear, it means that the skin is under continuous destruction. The best treatments should be adjusted according to the depth, location, size and the medical conditions of the wounds. In the period of inflammation and cell-proliferation, the efficiency of recovery, duration of the inflammation, and the final fate of the scars are determined mainly by various external factors and the condition of the wounds.
Applications of wound-dressings could prevent further impairments, such as pathogen infection, dehydration and excessive exudate. Traditional dry wound dressings, like cotton and gauze, have the ability to block external mechanical invasions, but could not provide an ideal environment for communication of leukocytes within the liquid and proliferation of epidermal cells. Furthermore, since dry wound dressings are easily adhesive to the wounds, it causes difficulty in changing and increases the duration of recovery and the potentials of permanent scars.
Moisture-retentive dressings have not been used for a very long time. In the 60’s, it was discovered that the period of recovery and possibility of permanent scar significantly reduced when the wound was in a moist environment. The moisture-retentive dressings not only elevate the cell proliferation rate, but also have many advantages, such as moisturizing and non-sticky texture, less difficulty in changing, and flexible dressing-size. Collagen, alginate, polyurethane foam, and hydrogel, are commonly used as moisture-retentive dressings.
Hydrogel is a three-dimensional structure with hydrophilic polymer chain, having the ability in absorbing water in great amount. Either physical or chemical cross-links could contribute to its three-dimensional structure. Naturally-occured collagen, chitin alginate and agarose could form hydrogel. Due to its splendid characters, hydrogel could be used in not only wound-dressings but also drug-delivers. Three components with high bio-capability are integrated to produce ideal wound-dressing. These components are poly (vinyl alcohol) with high water-content, dextran with the ability in promoting wound-recovery and chitosan with anti-microbial characteristic, when glutaraldehyde is used as the cross-linker.
In this study, 6% PVA hydrogel was chosen owing to its texture and further applications. In the anti-microbial test, 0.25% chitosan was found to provide the best anti-microbial ability. In the cell-toxicity test, it has shown that no residue of glutaraldehyde, which might undermine the growth of cells, was remained in our hydrogel-making procedure. In the cell-proliferation test, the greatest cell-proliferation happened to be on the hydrogel with 4% dextran. In the following physical analyses, the addition of chitosan and dextran was appeared to promote the thermo-stability, mechanical properties, water retention and moisturizing ability in the PVA hydrogel. In conclusion, the PVA/chitosan/dextran hydrogel has a promising potential as a wound dressing in the future.
| Identifer | oai:union.ndltd.org:TW/104NTU05406007 |
| Date | January 2016 |
| Creators | Tien-Ni Tseng, 曾天倪 |
| Contributors | Kai-Yin Lo, 羅凱尹 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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