碩士 / 國立臺灣科技大學 / 化學工程系 / 104 / In recent years, self-assembled monolayer (SAM) technology has
found a wide variety of applications in both nanotechnology and
biomedical fields. In this study , we used two kinds of sources to prepare
self-assembled films. One is the combination of hyaluronic acid and
insoluble cyanophycin, which was obtained by cultivating Escherichia coli
carrying BL21-CodonPlus (DE3)-RIL with Synechocystis sp. PCC6803
cphA. The other one is composed of poly-glutamic acid and insoluble
cyanophycin. Insoluble cyanophycin, containing arginine and lysine, is a
cationic polyelectrolyte, whereas poly-glutamic acid and hyaluronic acid
are anionic polyelectrolytes. Therefore, they can be adsorbed onto each
other due to electrostatic interactions. The film preparation was carried out
by two methods. One was co-precipitation method based on random
adsorption. The other one was layer-by-layer self-assembled method,
which was made of the deposition of alternating charged polyelectrolytes.
The changes in function groups were examined by FTIR (ATR). The
conjugates were analyzed by a colorimetric method, the Sakaguchi test, to
measure the contents of the insoluble cyanophycin. And scanning electron
microscopy (SEM) and atomic force microscopy (AFM) were used to
measure the surface roughness and morphology. The L929 cells and Hs68
fibroblasts were employed to test cell growth. The L929 cells were further
to examine the efficiency of cell migration for a wound recovery.
The results based on the colorimetric method showed that a high
amount of hyaluronic acid or (poly-glutamic acid) was associated with a
high amount of insoluble cyanophycin. The film thickness and roughness
IV
were better for the use of hyaluronic acid and insoluble cyanophycin. For
cell growth and cell migration, (hyaluronic acid)/ (insoluble cyanophycin)
by the co-precipitation method can produce better films compared to the
others. The results of insoluble cyanophycin content analyzed by the
colorimetric method in the films showed that the more cyanophycin
content in the films, the better condition for cell to grow.
| Identifer | oai:union.ndltd.org:TW/104NTUS5342107 |
| Date | January 2016 |
| Creators | JYUN-YIN SIAO, 蕭竣尹 |
| Contributors | Wen-Chi Tseng, 曾文祺 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 149 |
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