碩士 / 慈濟大學 / 生命科學系碩士班 / 104 / Phosphatase of regenerating liver-3 (PRL-3) is a protein tyrosine phosphatases (PTPs). Though the regulation of signal transduction pathways, members of PTPs family play important roles in cell proliferation and survival. Recently, PRL family proteins have been implicated in the process of cancer metastasis through its ability to promote cell proliferation and migration. However, little is known about their physiological role during embryonic development. Previously, we have shown that zebrafish PRL-3 is expressed in early embryonic development. To further address the developmental role of PRL-3 during embryonic development, we depleted the expression of PRL-3 by using morpholino antisense oligos. In comparison with control embryos, PRL-3 morphants presented an aberrant distribution of yolk syncytial nuclei (YSN) at 1K cells stage. During epiboly, a significant percentage of PRL-3 morphants present a phenotype of epiboly delay. Among these PRL-3 morphants, the premature formation of the actin-myosin ring can be observed. The embryonic phenotypes of PRL-3 morphants include the malformation of body axis and the embryonic death within 24 hours after the morpholino injection. In conclusion, our findings suggest that the zebrafish PRL-3 is essential for the embryogenesis and plays a crucial role in controlling the process of epiboly through the modulation of actin-myosin ring formation.
| Identifer | oai:union.ndltd.org:TW/104TCU00105006 |
| Date | January 2016 |
| Creators | WANG,TING-FANG, 王亭方 |
| Contributors | LIN,MING-DER, 林明德 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 57 |
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