碩士 / 東海大學 / 生命科學系 / 104 / Benthic diatoms are sensitive to physical and chemical factors in the freshwater ecosystem and are commonly used as biological indicators for monitoring river water quality changes. This study investigated the changes of the benthic diatom communities after three types of effluents and in order to elucidate the relationship between changes of the community and environmental factors, using microscopic observations and next generation sequencing of 18S rDNA. The application of diatom index was studied. Six mixed samples were collected from upstream and downstream sites of three selected discharge points at Donghai Bridge domestic point, Taichung Industrial Park and Taichung City Precision Machinery Innovation Technology Park within a span of five kilometers in Fazi River, Taichung. Diversity and community of diatoms were investigated by morphological diatom and used to calculated DAIpo, TDI, PTV and GI. DNA was extracted from the same samples and 18S rDNA of diatom was amplified and analyzed by 454 Pyrosequencing. Diversity data from microscopy and NGS were both used to analyze the relationship with environments by Redundancy Analysis. Using classic method, 17 genus and 42 species were recorded; whereas 33 genus and 164 species were obtained using molecular survey. Relationship between community similarity and geographic distance show that effluents influenced diatom community. Redundancy analysis show that some diatom were tolerant of endocrine disrupting chemicals and heavy metals and have environmental preference, however, the correlation was not significant. The variations in population of Navicula cryptocephala,Stauroneis phoenicenteron,Gomphonema parvulum, Nitzschia palea and N. subacicularis were correlated with concentration of endocrine disrupting chemicals and heavy metals. Diatom community index (DAIpo) and pollution tolerant valves (PTV) indicates the study sites of Fazi River were moderate pollution. Generic index (GI) showed serious pollution. Trophic diatom index (TDI) indicates the meso-eutrophic. These four indices demonstrate the ecological status of Fazi River.
| Identifer | oai:union.ndltd.org:TW/104THU00112004 |
| Date | January 2016 |
| Creators | Tzu-Jui Yeh, 葉子瑞 |
| Contributors | Pi-Han Wang, 汪碧涵 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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