碩士 / 東海大學 / 食品科學系 / 104 / Alzheimer’s disease (AD) is major caused by highly oxidative stress, which accelerates the amyloid β-peptide (Aβ) plaques and neurofibrillary tangles (NFTs) accumulated in brain. Pleurotus eryngii (PE) has been known as a rich source of antioxidants including ergothioneine, adenosine and polyphenol, which can reduce the aging phenomenon by oxidative stress in body. The aim of this study was to investigate the proximate compositions and bio-functional compounds in PE and evaluate the cognitive impairment of low (LPE), medium (MPE) and high (HPE) dosages of PE by Aβ induced-Alzheimer’s disease model in C57BL/6J mice. In proximate compositions, the contents of moisture, dry matter , crude ash, crude fat, crude protein and carbohydrate were 8.34 ± 0.14、91.66 ± 0.14、5.75 ± 0.02、1.55 ± 0.01、16.79 ± 0.11 and 67.60 ± 0.26%, respectively. Analysis of bio-functional components, the levels of ergothioneine, adenosine, total phenols and polysaccharide were 1.36 ± 0.05、3.07 ± 0.08、3.58 ± 0.13 and 223.89 ± 18.39 mg/g DB, respectively. The results of Alzheimer’s disease model by 6-week feeding of PE was showed that there is no adverse effects on body weight gain, food efficiency, serum biochemical parameters and histopathological examinations of liver and kidney in all dosages. The relative brain weight of Aβ group was significantly lower than that of other groups (p<0.05). It showed that PE could retard the atrophy of brain. In reference memory task, it showed that LPE、MPE and HPE significantly decreased percentage of escape latency (49-85%) and escape distance (53-69%) compared to that of Aβ group (p<0.05). With regards as the results of probe and T maze tasks indicate that PE administration potently ameliorated the memory deficit in mice. By AD pathology index analysis, PE can significantly decrease the contents of phosphorylated τ-protein, depositions of Aβ plaques, values of malondialdehyde and protein carbonyl in brain. These results suggest that PE can attenuate the memory and learning ability in Aβ-induced Alzheimer's disease mice and have great benefit to development as functional food on prophylaxis of AD.
| Identifer | oai:union.ndltd.org:TW/104THU00253004 |
| Date | January 2016 |
| Creators | HUANG,PO-CHANG, 黃柏璋 |
| Contributors | LIANG,CHIH-HUNG, CHIANG,SHEN-SHIH, 梁志弘, 蔣慎思 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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