碩士 / 大同大學 / 生物工程學系(所) / 104 / Davallia mariesii Moore ex Bak. is a slow-growing perennial medicinal fern. The rhizoma of Davallia contain with high antioxidants such as phenolic compound, triterpenes compound. A proliferation protocol of callus with high total phenolic content of Davallia mariesii Moore ex Bak by liquid culture has been established in this study. The rhizomes from sporophytes of D. mariesii cut into fine pieces were cultured in 1/2 Murashige and Skoog (MS) liquid media supplemented with different plant growth regulators combination of 2,4- dichlorophenoxy acetic acid (2,4-D), 6-benzyladenine (BA), thidiazuron (TDZ), gibberellic acid (GA), indolebutyric acid (IBA), and picloram. The green callus developed from tissue pieces when cultured in medium containing 4.54 μM TDZ and 0.9 μM 2,4-D has best result of 28 combinations after 30 days. The average growth rate was 2.5 times than the initial inoculation and total phenolic content was 31.8 μg gallic acid equivalent / mg DW. The average 1 g fresh weight of callus can regenerated approximately 1700 sporophytes after 4 months culture.
The best inoculation density for sporophytes proliferation in 1L bubble column bioreactor was 1 % compared with 0.6、2、3 %. The growth ratio of sporophytes was 158.63 % and total phenolic content was 24. 4 μg gallic acid equivalent / mg DW. The total phenolic content in sporophytes was increased to 34.8 μg gallic acid equivalent / mg DW when the medium salt ratio of NH4NO3 / KNO3 μM was changed to 10/20 NH4NO3 / KNO3.
| Identifer | oai:union.ndltd.org:TW/104TTU05106013 |
| Date | January 2016 |
| Creators | Yi-ting Xing, 謝亦婷 |
| Contributors | Chin-wen Ho, 何錦玟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 94 |
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