碩士 / 中華科技大學 / 健康科技研究所在職專班 / 105 / Acrylic acid was grafted to ozone treated alginate/polyvinyl alcohol (Alg/PVA) hydrogels. The resulting hydrogels were further grafted with chitosan (CS) via esterification. Afterward CS-grafted hydrogels were immobilized with hyaluronic acid (HA). The resulting Alg/PVA-HA hydrogels were characterized including the blood compatibility, the biocompatibility, and the cytotoxicity. These properties were employed to evaluate the applicability in biomedical areas.
Surface properties of modified Alg/PVA-HA hydrogels could be detected by X-Ray Photoelectron Sprectroscpoe (XPS) and dyeing. The effect of the positive charges of CS-grafted material on the absorption of negatively charged proteins of human serum albumin (HSA) and human plasma fibrinogen (HPF) was be studied. After grafting CS, the interaction between the membrane surface and the negatively charged proteins were electrostatic attraction. Thus the amount of adsorption/attachment increased with the surface positive charges on the hydrogels. One the other hand, the hydrogels immobilized with hyaluronic acid could decreased the amount of adsorption/attachment proteins. In addition, the in vitro cytotoxicity test results from culturing L929 fibroblasts show that these modified Alg/PVA-HA hydrogels were not cytotoxic.
| Identifer | oai:union.ndltd.org:TW/105CHIT1743002 |
| Date | January 2017 |
| Creators | CHOU, HSING-LING, 周杏玲 |
| Contributors | JAO, WIN-CHUN, 饒文娟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 54 |
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