碩士 / 嘉南藥理大學 / 保健營養系 / 105 / 5-Fluorouracil (5-FU) is the basic chemotherapeutic agent used to treat colorectal cancer, but it produces many side effects. The use of phytochemicals in combination with chemical drugs is expected to reduce side effects and improve survival of patient. Morinda citrifolia also known as Noni, has demonstrated that had antioxidant capacity and inducing apoptosis in tumor cells. The aim of this study was to investigate the effects of the supernatant of ethanolic extracts from Noni leaf (LES) and its fractions F1 and F3 (Rutin) combine with 5-FU on the cell viability, oxidative stress, and signaling transduction of colorectal cancer cell line, HT-29. The results showed that the treatment of HT-29 colon cancer cells with each samples and 5-FU (1:1, w/w) significantly all decreased cell viability. The 5-FU/F3 combination enabled a more effective inhibition of cell growth and the induction of apoptosis in HT-29 cells than 5-FU alone for 72 hours, followed by 5-FU/F1 for 48 hours and 5-FU/LES for 24 hours, according to the half inhibitory concentration (IC50) and combined index (CI) calculated by the combination-index methods of Chou and Talalay. The combination of F1 and 5-FU displayed a greater synergistic effect for 24 hour than the others combination. The combination of LES, F1 and F3 with 5-FU significantly inhibited the proliferation of HT-29 cells by increasing the p-β-catenin/β-catenin ratio and the expression of GSK3β, and promoting the apoptosis of HT-29 cells by increasing the activity of caspase-3, the expression of cleaved PARP and bax/bcl-2 ratio. Furthermore, the 5-FU/F3 combination could stimulate intercellular ROS accumulation in HT-29 cells. In conclusion, the results of this study suggest that chemotherapy using natural dietary agents with chemical agents represents a superior cancer treatment. Treatment of HT-29 cells with F3 or F1 combined with 5-FU is expected to achieve the best results. It is also possible to use LES as an auxiliary food to treat colorectal cancer cells because of lower price and accessibility.
| Identifer | oai:union.ndltd.org:TW/105CNUP0217009 |
| Date | January 2017 |
| Creators | TSAI, JING-HUA, 蔡靜華 |
| Contributors | CHEN, SHIH-YING, 陳師瑩 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 111 |
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