碩士 / 嘉南藥理大學 / 環境工程與科學系 / 105 / In this study, aerobic batch experiments were conducted in order to introduce a biological treatment instead of chemical treatment for contaminated harbor sediment of Kaohsiung harbor. The aim of the study was to investigate the possibility of mixed culturebacteria to remove COD and PAHs in the sediment by using a bacteria mixture prepared by the polluted-contaminated soil. The bacteria mixture consists of three specific diesel-degradable bacteria including Achromobacter insolitus, Candida spp., and Xanthobactor polyaromatici yorans which are isolated from diesel storage tank for diesel contaminated soil treatment. The experiments were carried out with 3 different experiments with/without synthetic nutrient solution and harbor sediment to investigate the COD removal efficiency of bacteria mixture and the microbes existed in sediment. In the end of each runs, the treated solution was sucked by using submerged MF hollow fiber membrane to retain and enrich the microbes inside of the reactor and then replaced with a new nutrient solution depending the experiment design.
The results showed that the mixed culturebacteria degraded SCOD (20.15%) and the highest PAHs removal was 86.19% of benzo[b]fluoranthene (BbF) in harbor sediment under aerobic condition. The microorganism in harbor sediment degraded SCOD (14.24±3.5%) and almost all PAHs, which the highest removal was 92.05 % of anthracene (An). The experiment with the nutrient solution addition removed high molecular PAHs, the removal of chrysene (Chr), benzo[b]fluoranthene (BbF), benzo[k]fluoranthene (BkF) was 21.29, 42.21, and 61.38%, respectively and SCOD removal was 41.66±14.24%.
The effect of cytotoxic of treated sediment on cell viability of normal cells (Clone 9) and cancer cells (HepG2), which the highest normal cells viability was 71.73% of sediment treatment by microorganism in harbor sediment and the highest cancer cells viability was 93.16% of treated sediment with mixed culture bacteria and nutrient solution.
The performances of mixed culture and indigenous bacteria were able to remove PAHs and SCOD from harbor sediment. In the future, microbial community should be studied on the analysis to understand the dominate microorganism for the degradation of organic-contaminated sediment.
| Identifer | oai:union.ndltd.org:TW/105CNUP0515018 |
| Date | January 2017 |
| Creators | SUDARAT JAISAARD, Sudarat Jaisaard |
| Contributors | CHANG, CHIA-YUAN, 張家源 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 118 |
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