碩士 / 輔英科技大學 / 生物科技系碩士班 / 105 / Microalgae are an important potential feedstock for biodiesel production, due to their ability to grow rapidly and to accumulate large amounts of storage lipids. However, the high temperature conditions in outdoor cultivation, especially during summer temperatures, inhibit the growth of many microalgal species. Thus, isolating microalgae strains with the ability to grow in the severe heat conditions, especially during summer temperatures, is of utmost importance. In this study, to obtain heat-tolant Chlorella strains for biodesel production by outdoor cultivation, Chlorella sp. ESP-31 was mutagenized with N-methyl-N'-nitro- N-nitrosoguanidine (NTG) and then grown at 40℃to select fast growing thermo-tolerant mutants. In total 30 of the isolated mutants, four of them, strain 89, 119, 305, and 314, were able to grow at 40℃. Additionally, when grown at 28℃ under 5 % CO2, the maximum biomass productivity of mutant 305, 314, 447, 455, and 456 were 0.78, 0.71, 0.79, 0.75, and 0.74 g/L/d, respectively, it was 22- 36% increased compared to 0.58 wild type 0.58 g/L/d. Nile red staining and microscope analysis revealed that five mutant strains (strain 305, 314, 447, 455 , 456) showed higher lipid content than wild type. In particular, strain 305 and 314 were able to grow at 40℃. These mutants with better biomass productivity, higher lipid content, and the the ability to grow at high temperature, they may be useful for microalgal biomass and biodiesel production by outdoor cultivation.
| Identifer | oai:union.ndltd.org:TW/105FY000111001 |
| Date | January 2017 |
| Creators | Siang-Di Song, 宋享廸 |
| Contributors | Te-Jin Chow, 周德珍 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 60 |
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