碩士 / 國立中興大學 / 生命科學系所 / 105 / Euryhaline brackish medaka, Oryzias dancena, is able to survive under wide ranges of environmental temperatures and salinities, and thus becomes an ideal experimental animal for environmental adaption research. In this study, seawater (SW)- and fresh water (FW)-acclimated brackish medaka were separately transferred into different low temperatures for one week and then RNA-sequencing (RNA-Seq) of the brain transcriptome was conducted. The RNA-Seq data were then used for de novo assembly, differential expression analysis, functional annotation and pathway analysis. The results of differential expression analysis showed that prl-1 and some other 13 unknown genes are significantly differentially expressed in the brain tissue of the FW/normal-temperature group and these genes could participate in the modulatory mechanisms of osmoregulation in brains of the brackish medaka. In addition, 45 common KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of these differentially expressed genes under different low temperatures are involved. Among them, the MAPK signaling, calcium signaling, neuroactive ligand-receptor interaction, and cell adhesion molecules are considered as the main pathways in brain under low-temperature stress. We also conducted a genome-wide selection pressure analysis between two closely related medakas, O. dancena and Oryzias latipes. According to the results of the analysis, 123 genes were under positive selection and only 5 of them were known. These candidate genes may provide valuable clues for medaka speciation researches. This study provides new insights into the regulatory mechanisms of fish in response to low temperatures of environments. The results can also be used as the basis of aquaculture industry in developing low-temperature adversity culture technology. Finally, a website (http://140.120.209.83/guan_test_templet/) based on the analyzed data of this study was also set up
| Identifer | oai:union.ndltd.org:TW/105NCHU5105035 |
| Date | January 2017 |
| Creators | Yu-Chen Huang, 黃俞禎 |
| Contributors | Tsung-Han Lee, Li-Ching Hsieh, 李宗翰, 謝立青 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 104 |
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