碩士 / 國立中興大學 / 食品暨應用生物科技學系所 / 105 / Alzheimer''s disease is a type of dementia that causes problems with memory, thinking and behavior. It is a chronic neurological dysfunction that symptoms usually develop slowly and get worse over time. The study showed that Alzheimer''s disease as a kind of neurodegenerative disease. The disease is related to the fibrous β-amyloid peptide (Aβ) accumulated in brain. Neprilysin (NEP) is a kind of enzyme in body which can degrade Aβ and prevent the occurrence of Alzheimer''s disease. However the level of NEP will decrease with age. The study showed that turmeric can increase the level of NEP and slow down the Aβ accumulation rate. Due to the poor bioavailability of turmeric, it is not easy to be absorbed by body. As a result, the physiological effect of turmeric is limited.
This study uses turmeric as material for Lactobacillus acidophilus and Lactobacillus plantarum fermentation. Obtain the supernatant after centrifugation and filtration, then use the cell model to explore the samples to know whether it can enhance the level of NEP protein and degrade the Aβ accumulation or not.
The result of bacteria screening showed that Lactobacillus acidophilus and Lactobacillus plantarum that fermented in turmeric grew better than other bacteria. Therefore, we treated the SH-SY5Y with fermentation supernatant, then used the Western blot and ELISA to analysis the the level of NEP and the Aβ-degrading activity respectively.
The result of NEP level by treated with different concentration fermention was showed that the fermented group is higher than Control and Vehicle. Furthermore, the L. acidophilus 10% is the highest than others.
Analysis the Aβ-degrading activity of SH-SY5Y treated with L. acidophilus 100%, L. acidophilus 10%, L. plantarum 10%, L. plantarum 100%, Vehicle, Control, we obtain the Aβ residue was 21830 pg/ml, 4194 pg/ml, 24436 pg/ml, 4648 pg/ml, 38497 pg/ml, 27966.7 pg/ml respectively. The results as above were far lower than Original group (172903 pg/ml). It also indicated that the LAB fermentation groups were lower than Control and Vehicle. Furthermore, the L. acidophilus 10% was the lowest. It showed L. acidophilus 10% had the best Aβ-degrading activity, as the same as the trend of NEP level result.
These results indicate the supernatant from Lactobacillus acidophilus and Lactobacillus plantarum that fermented with Curcuma longa can effectively enhance the Neprilysin protein expression and degrade the β-amyloid.
| Identifer | oai:union.ndltd.org:TW/105NCHU5253048 |
| Date | January 2017 |
| Creators | Yu-Ting Li, 李侑庭 |
| Contributors | Meei-Yn Lin, 林美吟 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 70 |
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