碩士 / 國立中興大學 / 生物產業機電工程學系所 / 105 / The purpose of this study was to use chlorophyll fluorescence monitoring in the development system for rotifer. The study analyzed the relationship between Rotifers- Nannochloropsis oculata mixed cultivation solution and chlorophyll fluorescence when cultivating a system for Brachionus rotundiformis, to realized the impact between of the rotifer’s colorless skin and absorption spectrum or chlorophyll fluorescence quenching to find a measurement of population density of rotifer during the progress. The best environment conditions for rotifer cultivation were set a temperature of 30°C, salinity 25 ppt, pH 7.5~8.5 and aeration with 45 ml/min.
The experiments set three different of initial feed densities, 800, 1,200 and 1,600×104 cells/ml. The results of experiments had demonstrated that a higher initial feed density, which initial food density is 1,600×104 cells/ml, obtained a greater egg ratio of the rotifer, had highest egg ratio with 0.754 eggs/female. The density at 27 hour after inoculation was 1667 individual /ml, which was up to the initial feed density of 2.38 times.
With the environment conditions of study, that were set a temperature of 30°C, salinity 25 ppt, pH 7.5~8.5 and aeration with 45 ml/min, we found if the rate of change of chlorophyll fluorescence had over 82.9 % during cultivating, it meant the cultivation system of rotifer approached the highest egg ratio period, the popular density of rotifer started increasing. And three different of initial feed densities, which means 800, 1,200 and 1,600×104 cells/ml, there rotifer density at 27 hour after inoculation were up to the density of highest egg ratio period of 1.49, 1.59 and 1.68 times, they all had great growth rate.
| Identifer | oai:union.ndltd.org:TW/105NCHU5415021 |
| Date | January 2017 |
| Creators | Cheng-Chih Liu, 劉承志 |
| Contributors | Chung-Chyi Yu, 尤瓊琦 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 61 |
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