碩士 / 國立成功大學 / 化學工程學系 / 105 / A microalgal lipid mixture rich in DHA was extracted from an isolated Thraustochytrium sp. DJ3 strain. This study aims to apply the microalgal lipid as delivery carrier of microalgae-extracted lutein in order to find a high value utilization of the microalgal lipid. In this study, the microalgal lipid was used to fabricate bilayered vesicles with negative charges by a forced formation approach. Furthermore, the vesicles were used as carriers for encapsulating microalgae-extracted lutein. Physical properties of the vesicles, such as initial size and zeta potential, are measured. Fluidity of the vesicular membranes with and without lutein was then investigated by infrared spectroscopy. The results showed that stable vesicles with negative charges could be successfully formed from the microalgal lipid with or without lutein. Microalgal lipid vesicles remained stable after being diluted to 5 mM and 2.5 mM. The existence of lutein had no effect on the initial size and zeta potential of the vesicles but slightly decreased the membrane fluidity of the vesicles. The existence of lutein had no adverse effect on the stability of the microalgal lipid vesicles, which showed the potential of using microalgal lipid for fabricating lutein-encapsulating vesicles. It was found that the encapsulation efficiency of lutein was about 41 %.
| Identifer | oai:union.ndltd.org:TW/105NCKU5063097 |
| Date | January 2018 |
| Creators | Hsin-YindYu, 余欣盈 |
| Contributors | Chien-Hsiang Chang, 張鑑祥 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 88 |
Page generated in 0.0079 seconds