碩士 / 國立交通大學 / 分子醫學與生物工程研究所 / 105 / Microalgae are large groups that can be found in sea, freshwater even in wastewater, they can divide into “Macroalgae” and “Microalgae” which based on their size. The study got wastewater from three pig farms and selected sixteen microalgae strains, we gave them name after molecular identification and phylogenetic tree. They are Chlorella sorokiniana, Micractinium sp., Chlorellaceae sp. and four groups Chlorella sp.; we found there are 8 strains might be new species and 1strain might be a new genus which named Chlorellaceae sp. strain P5 and we chose it as our potential microalgae. Microalgae can grow in phototrophy or heterotrophy which depends on their function, we found Chlorellaceae sp. strain P5 can grow in wild range at 20℃to 40℃, adapt to pH4 to pH9 and tolerate 0% to 3% salinity, most importantly, it can grow in heterotrophic medium. Although phototrophy has least cost, the biomass is lower and the culture time is too long than heterotrophy. We found P5strain biomass prodictivity can raise from 0.0261±0.003 to 1.615±0.047 after culture from photoprophy to optimized heterotrophy and it increased 61.877 fold. In recent years, microalgae are as a new source of biofuel, because they provide higher biomass than comparable energy crops and act as a low-cost, high-energy and entirely renewable feedstock; some microalgae might contain higher amino acid that can add to animal feed. On the other hand, livestock wastewater contain high level inorganic matter, like nitrogen and phosphorous concentrations, these can cause severe environmental problems. The main fatty acids in Chlorellaceae sp. strain P5 are C16 and C18, these also main fatty acid used in biodiesel; the amino acid compositions are multivariate, and it had potential add to animal feed; Chlorellaceae sp. strain P5 also can grow in diluted twice and fourfold piggery drainage tank wastewater, it can remove 80% nitrate, 98% ammonia and 46% phosphorus after cultured 1 week.
| Identifer | oai:union.ndltd.org:TW/105NCTU5538002 |
| Date | January 2016 |
| Creators | Chiang, Yin-Ling, 江尹玲 |
| Contributors | Tseng, Ching-Ping, Liaw, Li-Ling, 曾慶平, 廖麗玲 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 97 |
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