碩士 / 國立高雄海洋科技大學 / 水產食品科學研究所 / 105 / Squid is important catches in Taiwan's offshore fishing ranked third in the world catch of squid. However, the squid processing will produce a lot of waste, such as viscera and ink sac that were discarded or as byproducts. Among them, squid ink (SI) accounted for about 1.3% of body weight has fish stench and is difficult to utilize. Some reports had been revealed that Cephalopods ink contained many bioactive substances, such as antioxidant, antibacterial and antitumor activity. Therefore, the antioxidant, antibacterial, angiotensin-I-converting enzyme (ACE) inhibitory activity and antitumor effects of neon flying squid (Ommastrephes bartramii) SI extracts extracted by methanol, ethanol, hexane and acetone as well as SI polysaccharide were evaluated. In ACE inhibitory activity, the SI extracts had no or very low ACE inhibitory activity (<0.2%), except for 1000 ppm of SI polysaccharide (SIP) with better ACE inhibitory activity (15.98%). No antibacterial activity against food pathogens was observed in all SI extracts. Cytotoxicity assay was performed using MTT assay on HeLa cell line and HL-60 cell line (Human promyelocytic leukemia cells). The cell viability of HeLa cells treated with SIP and SI methanol or ethanol extracts was decreased and the highest inhibitory rate reached 30% at SIP treatment. However, no or very low inhibitory activity on HL-60 cells treated with SIP and SI methanol or ethanol extracts was observed. In antioxidant activity, except for hydroxyl radical scavenging ability, the SIP showed higher antioxidant ability in scavenging DPPH, ABTS radical scavenging, and ferrous ion-chelating ability than SI methanol and ethanol extracts. When the SIP was separated using ultrafiltration into two fractions (<5 KDa and >5 KDa), the fractions with MW >5 KDa showed to elevate DPPH radical scavenging activity and hydroxyl radical scavenging ability. The SIP contained 65.03% total sugar, 0.14% protein, 0.1% lipid, 11.36% ash, 14.24% uronic acid, and 1.84% sulfate.
| Identifer | oai:union.ndltd.org:TW/105NKIM0084017 |
| Date | January 2017 |
| Creators | JHOU, AN-CI, 周安琪 |
| Contributors | Yung-Hsiang Tsai, Yi-Chen Lee, 蔡永祥, 李憶甄 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 116 |
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