碩士 / 國立高雄海洋科技大學 / 海洋生物技術研究所 / 105 / In this study, six novel genus and species bacteria isolated from water environments in Taiwan were subjected to identification, classification and nomenclature.
A bacterial strain designated LSN-49T was isolated from a brackish river in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain LSN-49T are gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of monopolar flagellum, non-spore forming, straight rods and forming shiny and translucent colonies. Growth occurred at 20-40 °C (optimum, 25-30 °C), at pH 6-10 (optimum, pH 7-8) and with 0-3 % (w/v) NaCl [optimum, 0-1 % (w/v)] tolerance. The predominant fatty acids are summed feature 3 (comprising C16:1ω7c/C16:1ω6c), C17:1ω8c and C16:0. The polar lipid profile consists of a mixture of phosphatidylethanolamine (PE), phosphatidylglycerol (PG), two uncharacterized aminophospholipids (APL1 and APL2) and four uncharacterized lipids (L1-L4). The major polyamine is putrescine. The major isoprenoid quinone is Q-8 and the DNA G+C content is 51.0 mol%. Phylogenetic analyses based on 16S rRNA gene sequences show that strain LSN-49T forms a distinct lineage with respect to closely related genera in the family Pseudoalteromonadaceae. Strain LSN-49T is most closely related to Pseudoalteromonas, Algicola and Psychrosphaera and show 89.3-93.0 % sequence similarity with members of the family Pseudoalteromonadaceae with validly published names. On the basis of the genotypic and phenotypic data, strain LSN-49T represents a novel genus and species of the family Pseudoalteromonadaceae, for which the name Salsuginimonas clara gen. nov., sp. nov. is proposed. The type strain is LSN-49T (=BCRC 81005T =LMG 29726T =KCTC 52439T).
A bacterial strain designated AHQ-12T isolated from a freshwater lake in Taiwan was characterized using a polyphasic taxonomy approach. Cells of strain AHQ-12T are gram-staining-negative, aerobic, non-motile, non-spore forming, straight rods and forming translucent white-colored colonies. Optimal growth occurred at 20 °C, pH 6.0 and 0 % NaCl. The predominant fatty acids are summed feature 3 (comprising C16:1ω7c/C16:1ω6c) and C16:0. The major isoprenoid quinone is Q-8 and the DNA G+C content is 50.4 mol%. The major polar lipids are phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized aminophospholipids and lipids. The major polyamine is cadaverine. 16S rRNA gene sequence analysis demonstrated that this isolate was unique, showing less than 91 % sequence similarity to its closest relatives, including members of the genera Ralstonia (89.7-90.8 %), Cupriavidus (88.8-90.3 %), Polynucleobacter (88.2-89.5 %), Burkholderia (86.6-90.3 %) and Pandoraea (89.2-90.1 %). Phylogenetic analyses demonstrated that strain AHQ-12T forms a distinct clade closely related to species of the family Burkholderiaceae. On the basis of the phylogenetic inference and phenotypic data, strain AHQ-12T should be classified as a novel species of a new genus in the family Burkholderiaceae, for which the name Formosimonas limnophila gen. nov., sp. nov. is proposed. The type strain is AHQ-12T (=BCRC 80690T =LMG 27847T =KCTC 32501T).
A bacterial strain designated KMB9T was isolated from a freshwater pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of strain KMB9T are gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of monopolar flagellum, non-spore forming, rods surrounded by a thick capsule and forming white-colored colonies. Growth occurred at 20-40 °C (optimum, 25-37 °C), at pH 6.5-7.5 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences show that strain KMB9T forms a distinct phyletic line within the order Burkholderiales, with less than 93.3 % sequence similarity to its closest relatives with validly published names. The predominant fatty acids are summed feature 3 (comprising C16:1ω7c/C16:1ω6c), C16:0 and C18:1ω7c. The major isoprenoid quinone is Q-8. The major polyamine is putrescine. The polar lipid profile revealed the presence of phosphatidylethanolamine, phosphatidylglycerol and several uncharacterized aminophospholipid, aminolipid, phospholipid and lipid. The genomic DNA G+C content of strain KMB9T is 54.5 mol%. On the basis of the genotypic and phenotypic data, strain KMB9T represents a novel species of a new genus in the order Burkholderiales, for which the name Parvibium lacunae gen. nov., sp. nov. is proposed. The type strain is KMB9T (=BCRC 81053T =LMG 30055T=KCTC 52814T).
A bacterial strain designated Sty a-1T, isolated from a reef-building coral Stylophora sp., collected off coast of Southern Taiwan, was characterized using the polyphasic taxonomy approach. Cells of strain Sty a-1T are gram-staining-negative, aerobic, poly--hydroxybutyrate accumulating, motile by means of flagella, non-spore forming, straight rod-shaped and colonies were yellow and circular. Growth occurred at 15-40 °C (optimum, 30-35 °C), at pH 6-10 (optimum, pH 6.5-8) and with 0-7 % NaCl (optimum, 2-3 %). The predominant fatty acids are iso-C15:0, iso-C17:1ω9c, summed feature 3 (comprising C16:1ω7c/C16:1ω6c) and iso-C17:0. The major isoprenoid quinone is Q-8 and the DNA G+C content is 68.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an uncharacterized aminophospholipid and three uncharacterized lipids. The major polyamines are spermidine, putrescine and homospermidine. Phylogenetic analyses based on 16S rRNA gene sequences show that strain Sty a-1T forms a distinct lineage with respect to closely related genera in the family Lysobacteraceae, most closely related to Lysobacter, Silanimonas, Arenimonas and Luteimonas and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 95 %. On the basis of the genotypic and phenotypic data, strain Sty a-1T represents a novel genus and species of the family Lysobacteraceae, for which the name Coralloluteibacterium stylophorae gen. nov., sp. nov. is proposed. The type strain is Sty a-1T (=BCRC 80968T =LMG 29479T =KCTC 52167T).
A bacterial strain designated Eup a-2T, isolated from a torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-2T are gram-staining-negative, aerobic, motile with flagella, poly-β-hydroxybutyrate- containing, rod-shaped and forming translucent colonies. Optimal growth occurred at 25 °C, pH 6-8, and in the presence of 2-4 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences show that strain Eup a-2T belongs to the genus Litoribrevibacter and show the highest levels of sequence similarity with respect to Litoribrevibacter albus Y32T (97.8 %). Strain Eup a-2T contained summed feature 3 (C16:1ω7c/C16:1ω6c) and C16:0 as the predominant fatty acids. The predominant isoprenoid quinone is Q-8. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphophatidylglycerol. Genomic DNA G+C content of strain Eup a-2T is 49.1 mol%. The DNA-DNA hybridization value for strain Eup a-2T with Litoribrevibacter albus Y32T is less than 30 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup a-2T should be classified as a novel species of the genus Litoribrevibacter, for which the name Litoribrevibacter euphylliae sp. nov. is presented. The type strain is Eup a-2T (=BCRC 81004T=LMG 29725T=KCTC 52438T).
A bacterial strain designated Eup a-8T, isolated from a torch coral Euphyllia glabrescens, was characterized using a polyphasic taxonomy approach. Cells of strain Eup a-8T are gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly--hydroxybutyrate-containing, rod-shaped and forming white colonies. Optimal growth occurred at 25-30 °C, pH 7-8, and in the presence of 2 % NaCl. Phylogenetic analyses based on 16S rRNA gene sequences show that strain Eup a-8T belongs to the genus Thalassotalea and show the highest levels of sequence similarity with respect to Thalassotalea ganghwensis JC2041T (97.1 %). Strain Eup a-8T exhibited 16S rRNA gene sequence similarity values of 94.9-97.1 % to the type strains of species of the genus Thalassotalea. Strain Eup a-8T contained C17:1ω8c, summed feature 3 (C16:1ω7c/C16:1ω6c), iso-C14:0 and iso-C16:0 as the predominant fatty acids. The only isoprenoid quinone is Q-8. The major polar lipids are phosphatidylethanolamine and phosphatidylglycerol. Genomic DNA G+C content of strain Eup a-8T is 41.5 mol%. The DNA-DNA hybridization value for strain Eup a-8T with Thalassotalea ganghwensis JC2041T is less than 39 %. Differential phenotypic properties, together with the phylogenetic inference, demonstrate that strain Eup a-8T should be classified as a novel species of the genus Thalassotalea, for which the name Thalassotalea coralli sp. nov. is presented. The type strain is Eup a-8T (=BCRC 80967T=LMG 29478T=KCTC 52169T).
| Identifer | oai:union.ndltd.org:TW/105NKIM0270007 |
| Date | January 2017 |
| Creators | Xie, Pei-Bei, 謝姵蓓 |
| Contributors | Sheu, Shih-Yi, 許世宜 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 246 |
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