Studies on the effect of bacteriocin-like substance producing Chromobacterium aquaticum on immune against disease infection in zebrafish (Danio rerio) / 產類細菌素之水生色桿菌對斑馬魚之免疫調節與疾病抵抗研究

碩士 / 國立屏東科技大學 / 生物科技系所 / 105 / Aquaculture is an important industry in the world. However, bacteria-induced diseases such as septicemia induced by Aermonas
hydrophila, often result in severe economic loss in aquaculture. Probiotics in one of strategies for preventing disease in aquaculture. Laboratory previous study , the bacteria was isolated from the lake and named as Chromobacterium aquaticum according to 16s RNA sequencing and biotechemistry analysis. After four days of culture, the supernatant has antibacterial activity. The antimicrobial activity in supernatant were remained in the presens of different temperature (40~100℃) and pH (2~11).Dietary supplementation of the c.aquaticum for one month increase IL、IL、IL、F-、F-kB、Lysozyme、c3b expression in whole body;IL、IL、IL、F-、F-kB、Lysozyme、c3b expression in intestine of zebrafish. The survival rate of A. hydrophila-infection zebrafish was increase after Chromobacterium aquaticum administration. This result to demonstrate that Chromobacterium aquaticum can be used as a potential probiotic in aquaculture for diseae control.

Identiferoai:union.ndltd.org:TW/105NPUS5111014
Date January 2017
CreatorsYi, Che-Chun, 衣哲均
ContributorsHu, Shao-Yang, 胡紹揚
Source SetsNational Digital Library of Theses and Dissertations in Taiwan
Languagezh-TW
Detected LanguageEnglish
Type學位論文 ; thesis
Format52

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