碩士 / 國立屏東科技大學 / 生物科技系所 / 105 / Pathogens induced disease cause significant stock losses in aquaculture. Most of the fish disease are caused by bacterial pathogens. Antibiotics are commonly applied to treat the disease in aquaculture, however the use of antibiotics for aquatic animals may not only lead environmental pollution problems but also can affect human health due to drug residues and the development of resistant strains of pathogens. Antimicrobial peptides (AMPs) are an important component of host defense. AMPs can damage or interfere with the cell membranes of pathogens and regulate immune responses against pathogens infection. Hepcidin is a small bioactive peptide with dual roles as an antimicrobial peptide (AMP) and as the principal hormonal regulator of iron homeostasis. In the present study, an expression plasmid in which hepcidin gene fused with EGFP was driven by zebrafish oocyte-specific ZP3 promoter and used to establish Tg (Zp3:hepc) transgenic zebrafish. The expression of recombinant hepc-EGFP protein in F2 fertilized eggs were confirmed by RT-PCR and Western blot. The result shown adult zebrafish fed with hepc-expressing fertilized eggs for 3 months exhibited higher expression level of immune response genes such as IL-1β, IL-6, IL-10, IL-15, TNF-α, COX-2, NF-ĸB, C3b, and TLR-4α compared to those in control group suggesting hepc-expressing fertilized eggs used as diet supplementation can enhance immunity against disease infection.
| Identifer | oai:union.ndltd.org:TW/105NPUS5111019 |
| Date | January 2017 |
| Creators | Ridha Handriany Danata, 李丹華 |
| Contributors | Shao-Yang Hu, 胡紹揚 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | en_US |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 49 |
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