碩士 / 國立屏東科技大學 / 動物疫苗科技研究所 / 105 / The main subject of this study is to develop a candidate vaccinein grouper andinvestigateprotective efficacy.By using epidemiological analysis,Vibrio harveyiisolates were collected from clinical cases in farmed grouper in Taiwan. 33 strainsV. harveyi isolates were divided into 18 pulsotypes(S1 to S18)by pulsed field gel electrophoresis (PFGE) with restriction enzyme (Sma I). Moreover, isolates were also subtyping into two types (VA and VB) with V. harveyi virulence factors and 2 types (RA, and RB) with antibiotic resistance genes.The targets of virulence factors and antibiotic resistance genes are reversible toxin 1 (VH1), 2 (VH2), 3 (VH3), 4 (VH4), hemolysin coregulated protein (Hcp), phage coat protein (Zot), Chloramphenicol (cat I, cat Ⅱ, catA2), Florfenicol (pp-flo) and Oxytetracycline (tetB and tet D)resistance gene. Withthe classification of virulence factors,an isolates (AOD99026-2Skin-5) were identified. The candidate vaccine, AOD99026-2Skin-5, was then mixed with adjuvant ISA763A to test vaccine efficacy. Immunization was performed by intraperitoneal injection (I.P.) and boostered at 14 days after immunication. The challenged dose of V. harveyi is 108CFU /fish. Mortalities were recorded at 21 days after challenge and the relative percentage survival (RPS) were calculated. The RPS at 30 days and 90 days were 97.6％ and 86.4％, and the agglutination titers were higher than control group. Taken together, the formalin-killed V. harveyi vaccine could be an effective vaccine directed against V. harveyi infection in grouper.
| Identifer | oai:union.ndltd.org:TW/105NPUS5290002 |
| Date | January 2017 |
| Creators | E, Ya-Chen, 鄂雅真 |
| Contributors | Chen, Shih-Chu, 陳石柱 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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