碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 105 / Two species of Coscinodiscophyceae, Skeletonema costatum and Thalassiosira weissflogii were assayed the effects of operation factors of cold storage or cultivation and quality maintenance at room temperature, aimed at the establishment of stable production of high quality food organism. This study was divided into three directions, isolation and purification, algae culture at room temperature, and cold storage.
The best way of algae isolation is to use the capillary method to collect the algae, filtrated and remove the impurity in the water will improve the quality of purification. The results showed that the best salinity was of daily growth rate was 26 ‰ (0.531 ± 0.006), and 29 ‰ (0.514 ± 0.003) was the second best, optimum salinity range was 26 ~ 29 ‰ in S. costatum. The best salinity of T. weissflogii was also 26 ‰ (0.354 ± 0.003), while the growth rate of 23 ‰,26 ‰,29 ‰ treatment was not significantly different, so the optimum range of salinity was between 23 and 29 ‰. The results showed the growth rate of 25 ℃ treatment group was higher than the 20℃. The best growth rate of all treatment in two diatoms was treated with 5000 lux, photoperiod 10L: 14D in 26‰ salinity medium at 25℃.
The results showed that the two diatoms could be preserved in Walne medium with 8℃ in the dark more than one month, and avoid stored the algae in declining phase. The concentrated of S. costatum. will die quickly, that was useless in cold-storage method, while the concentrated of T. weissflogii was efficient. The excess nutrients of medium will cause algae death, added to the standard Walne medium is limited. The treatment of S. costatum preserved more than three months , which cultured in 6-7 days at 25℃,that was the most suitable conditions for cold storage.
| Identifer | oai:union.ndltd.org:TW/105NTOU5086021 |
| Date | January 2017 |
| Creators | Yang, Meng-Lin, 楊孟霖 |
| Contributors | Huang, Yii-Shing, 黃沂訓 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 58 |
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