碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 105 / Pacific white shrimp (Litopenaeus vannamei) is one of the major cultured crustaceans around the world. Female has higher growth rate and size compared with male. Therefore, monosex culture of female could increase production yield greatly. In most malacostraca crustaceans, sex differentiation is controlled by a male specific organ, androgenic gland (AG). In this study, three tissues (terminal ampule with AG attached, testis and ovary) were used to generate the cDNA library through the transcriptome analysis. 13 sex-dimorphic genes (FPKM>32 in testis/ovary, <0.213 in ovary/testis) were selected to analyze the expression profile through the qPCR analysis. One of the female-specfic gene, contig223, had exclusively expression in ovary compared to other tissues. Based on in situ hybridization data, contig223 was localized in the primary oocyte but not the oogonia. In addition, a key gene for male differentiation in most malacostraca crustacean, IAG was selected to understand the mechanism of sex differentiation in shrimp. according to qPCR data, IAG was highly expressed in the terminal ampule of vas deferens. In situ hybridization staining shows that IAG was localized in AG cell attached to the terminal ampule. In contrast, unlike Macrobrachium rosenbergii, several tissues also had IAG expression. Furthermore, IAG expression did not change in IAG-expressed tissues after the ablation of AG. Thus, our results find an ovary-specific gene (contig223) in the female shrimp. Our data also demonstrates that AG-removal male shrimp wasn’t to alter the sexual fate from male to female. Talking together, the mechanism of sex differentiation may had highly diversity between penaeid shrimp and palaemonid shrimp.
| Identifer | oai:union.ndltd.org:TW/105NTOU5086036 |
| Date | January 2017 |
| Creators | Cheng, Hao-Sheng, 鄭顥生 |
| Contributors | Chang, Ching-Fong, Wu, Guan-Chung, 張清風, 吳貫忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 91 |
Page generated in 0.0015 seconds