碩士 / 國立臺灣海洋大學 / 水產養殖學系 / 105 / Accessory nidamental glands (ANG) is the accessory reproductive gland of female in Sepioteuthis lessoniana (oval squid). Symbiotic bacteria growth is associated with ovarian development and ANG contain colorful pigments in mature female. The objective of this study was to investigate that the communication between the host (ANG) and symbiotic bacteria during ovary growth. However it is difficult to identify ANG development by histology. To solve this problem, the ovary developmental stage was used as an assistance. To find the marker genes for ovarian development stages, ovarian transcriptomic data was used to select candidate genes. Real time PCR (qPCR) was used to confirm the gene expression profiles in different ovarian stages. To understand the bacterial selection in the ANG (host), amplicon analysis was used to classified the variety of bacterial community structure during ANG development. Furthermore, transcriptomic analysis was used to understand the molecular mechanism of ANG-bacteria communication by ANG. According to the histological data, the bacteria clonies were observed in the ANG while the follicle cells penetrated into the oocytes. The colonies of symbionts were observed the yellow and red pigments in the ANGs while oocytes entered vitellogenic stage. Based on the gene expression profiles (qPCR analysis), several genes was selected as the marker for different ovary stages. These genes include bmp2 (bone morphogenetic protein type II) and bmpr1b (bone morphogenetic protein receptor type-1B) as the folliculogenetic marker), vtg1 and vtg2 (vitellogenin type 1 and type 2) as the vitellogenetic marker, apoLp-long and apoLp-short (apolipophorin long isoform and short isoform) as the mature follicle (before ovulation) marker. According to amplicon analysis, the bacterial population are strongly selected by ANG. However, the dominant bacteria showed defference in the different collection periods (year 104~105: Alphaproteobacteria unclassified, year 105~106: Rhodobacteria). Taking together, our datas demonstrates that ANG developmental stage are highly associated to ovarian growth. The bacteria population are selected by host. To clarify the mechanism of host-bacteria communication, we need to identify the characteristics of target bacteria in next step.
| Identifer | oai:union.ndltd.org:TW/105NTOU5086037 |
| Date | January 2017 |
| Creators | Chen, Chih, 陳稚 |
| Contributors | Chang, Ching-Fong, Wu, Guan-Chung, 張清風, 吳貫忠 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 105 |
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