碩士 / 國立臺灣海洋大學 / 食品科學系 / 105 / Recent reports have revealed that fucoidan has osteoconductive property, which can elevate osteoblast-related gene expressions. Another well-known candidate for bone tissue engineering applications is hydroxyapatite (HAP). The characteristics of hydroxyapatite, including size, shape, and surface charge, are the primary parameters for cellular uptake. Therefore, the aim of this study is surface modification of hydroxyapatite nanoparticles (n-HAP-N) and hydroxyapatite microparticles (μ-HAP-N) to combine with fucoidan, and then to encapsulate melatonin for evaluating its effect on differentiation of murine osteoblast-like cell line (MC3T3-E1). Hydroxyapatite nanoparticles and microparticles were 20 nm and 5 μm. in the diameter. Fourier-transform infrared spectrometer (FT-IR) and Ninhydrin test were used to confirmed the adsorption and binding of the 12-aminododecanoic acid on the HAP surfaces. Elemental analyzer calculated fucoidan grafting content of nano and micro hydroxyapatite- fucoidan complexes (HAP-N-F), which were 7 μg/mg and 15 μg/mg. X-ray diffraction (XRD) revealed that surface modifications did not change the crystal structure of HAP and successfully encapsulate melatonin. Complexes with dosage 1 mg/mL co-cultured with pre-osteoblast cells showed good biocompatibility. The results showed that added fucoidan to n-HAP-N and μ-HAP-N can enhance alkaline phosphatase (ALP), among which μ-HAP-N-F showed greater ALP activity. Melatonin-loaded hydroxyapatite-fucoidan complexes (HAP-N-F/M) can increased ALP activity and enhanced calcium deposit by releasing melatonin. In summary, melatonin-loaded hydroxyapatite-fucoidan complexes were successfully synthesized and could be a potential candidate for bone-tissue engineering applications.
| Identifer | oai:union.ndltd.org:TW/105NTOU5253072 |
| Date | January 2017 |
| Creators | Yang, Tzu-Wei, 楊子薇 |
| Contributors | Huang, Yi-Cheng, 黃意真 |
| Source Sets | National Digital Library of Theses and Dissertations in Taiwan |
| Language | zh-TW |
| Detected Language | English |
| Type | 學位論文 ; thesis |
| Format | 67 |
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